The Research Of Late-onset Alzheimer's Disease With Rs5984894 On PCDH11X

[Backgroud] Alzheimer's disease (Alzheimer's disease, AD) is a progressive decline of intelligent comprehensive neurodegenerative diseases. Now the disease is a high threat to health of the elderly diseases.Mild cognitive impairment (Mild cognitive impairment, MCI) is a memory reduced status which is not include into the AD and health.It has recently been included into the DSM-V sub neurocognitive dysfunction syndrome.Current studies suggest that MCI is a transitional stage of AD and health cognitive.Now that Alzheimer's disease is a polygenic disease and influenced by the surrounding environment.With Minerva, who in 2009 discovered the multiple gene loci of Protocadherin 11X (PCDH11X) (especially rs5984894) had significant difference associated with LOAD.Lescai and Beecham Research on the European race and our study found the North Han were unable to confirm the research findings with Minerva.Research PCDH11X for MCI and no one at home and abroad involving. So, the purpose of this study is to explore the conflict points of LOAD and PCDH11X's correlation and the new starting point of MCI and PCDH11X's correlation.[Objective] According to the report that the mutate in PCDH11X rs5984894 loci is significantly related with LOAD in 2009, we repeat that on the Yun Nan Han.[Methods] To 84 control cases respectively,80 cases of patients with LOAD normal controls were and 76 cases of mild cognitive impairment>65 years old people. The LOAD group had 80 patients, male 32, female 48 cases (mean age 72.51±6.32 years).The normal group had 84 patients,51 male and 33 females, (mean age 72.19±6.03 years).The MCI group had 76 patients,43 male and 33 females (mean age 72.26±5.47 years).Through the PCR technology and extinction enzymes technology on the PCDH11X rs5984894 loci mutations contrast study was carried out. SPSS16.0 statistical software used for data analysis using gene counting method gene frequencies and genotype frequencies and tests their compliance with the Hardy-Weinberg law of genetic equilibrium. Mean value were analyzed using t test. Between the three groups for age, sex, allele frequency, genotype frequency interaction was analyzed by variance test, for those with missing variance rank test. Severity of disease and genotype, gender distribution was analyzed by chi-square test or exact method. All statistical tests are P<0.05 was considered statistically significant. Measurement data with mean±standard deviation (x±s) said.[Results] Control group and mild cognitive impairment (mild cognitive impairment, MCI) group, LOAD patient group rs5984894 mutation was no significant difference (P> 0.05). LOAD groups of different genotypes HAMD, ADAS-cog in each group no significant difference, but among MMSE every group in addition to immediate recall group (P<0.05), other groups were not statistically different (P> 0.05). HAMD, ADAS-cog, MMSE genotypes in each group no significant difference between sex (P> 0.05). HAMD with the extent of the disease LOAD groups was not statistically significant (P> 0.05), but the ADL scores were statistically significant differences (P <0.01). The genotype distribution of the different severity of disease was no significant difference (P> 0.05). Different severity of the genotype distribution between the genders there was a significant difference. (P<0.05)[Conclusion] Rs5984894 PCDH11X gene locus mutation and the incidence in Southern Chinese Han may not be associated LOAD. The mutation sites of these symptoms associated with immediate recall, memory of those mutations was significantly lower scores than those without mutation. rs5984894 Mutation may be more women than men.