| Objective To investigate the value of combined detection of 3 serum tumor markers CA125, SMRP and IL-17 in early diagnosis of epithelial ovarian cancer. Methods Preoperative fasting serum IL-17 and SMRP of epithelial ovarian cancer patients was detected using Elisa method in strict accordance with the instructions kit. After the reaction termination, the absorbance of the holes in 450nm was read. Using the OD value of the standard material holes as vertical axis, concentration as abscissa, a standard curve was drawed and the straight line equation was calculated by ELISA software. Linear regression of the sample with the expected concentration correlation coefficient R were big than 0.95, then calculate the corresponding SMRP concentrations of IL-17 according to the sample OD value. Serum levels of IL-17 applied radioimmunoassay. Results The positive rate of alone detection CA125 in early diagnosis of ovarian cancer was 45.00%, and 85.71% in advanced patients; the positive rate of IL-17 in early diagnosis of ovarian cancer was 60.00%, and 90.48% in advanced; the positive rate of SMRP in early diagnosis of ovarian cancer was the highest with 70.00%, and 85.71% in advanced. SMRP in early diagnosis of ovarian cancer revealed the highest positive rate, and CA125 showed the lowest positive rate. With the increase of the joint species of tumor markers from 2 to 3, the positive rate increased to 95.00%, and 100.00% in advanced.Sensitivity of single detection increased from 65.85% to 97.56%, and negative predictive value increased to 99.00%. However, combined detection can not improve the accuracy, specificity and positive predictive value. The positive rate of 3 tumor markers increased accordingly with the clinical stage, particularly in CA125. Conclusion Combined detection of tumor CA125, SMRP and IL-17 could increase the diagnostic sensitivity of ovarian cancer; it displayed an important reference value in the diagnostic of epithelial ovarian cancer. |
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