The Expression Of CXCL12 And CXCR4 In Early Gastric Canccer And Advanced Gastric Cancer

Objective To investigate the expression of chemotactic factor CXCL12(SDF-1) and its receptor CXCR4 in early gastric carcinoma and advanced gastric carcinoma which include peritoneal metastasis,to provid important indicators for revealing the molecular mechanise of gastric carcinoma.Methods Immunohistochemistry and RT-PCR were used to test the expression of CXCL12/CXCR4 protein and mRNA in normal gastric mucosa tissue, early gastric carcinoma and advanced gastric carcinoma.Results 1. Immunohistochemistry showed:21 cases of normal gastric tissue were not express CXCL12 and CXCR4;CXCL's expression rate in early gastric carcinoma was 50%(5/ 10), CXCR4's expression rate was 30%(3/10), there was statistically significant (p≤0.05).In advanced gastric carcinoma group (excluding peritoneal metastasis) CXCL12's expression rate was 90%(18/20), CXCR4's expression rate was 75%(15/20), and there was statistically significant with normal gastric tissue and gastric cancer(p≤0.05)..In advanced gastric carcinoma with peritoneal metastasis. CXCL12 and CXCR4's expression rate was 100%(9/9), slightly higher than that of in advanced gastric carcinoma group (excluding peritoneal metastasis)(p>0.05), There was no significant difference. From the point of view of the level of mRNA, CXCL12/CXCR4 did not express in normal gastric tissue, but in early and advanced gastric carcinoma were expressed, and the expression in early gastric carcinoma was weak, in advanced gastric cancer was high, there was statistically significant (p≤0.05).2. CXCL12/CXCR4 were significantly related with TNM staging of gastric carcinoma and lymph node metastasis and peritoneal metastasis,3.The expressions of CXCR4 and CXCL12 had relativity(p≤0.05). Conclusion 1. CXCL12/CXCR4 had important biological role in maintaining further proliferation of gastric cancer cells, and promoting the invasion and metastasis of gastric carcinoma.2. CXCR4 and CXCL12 have some correlations between the expression, suggesting that high expression of CXCR4 in cancer cells, may CXCL12 chemokine, traction to accelerate the evolution of gastric carcinoma.