Protective Effect Of Ethanolic Extract Of Zingiber Officinale On HepA Tumor In Mice

The zingiber officinale (ZO) is a kind of plant having yellowish-green flowers and a pungent, aromatic rhizome. It has been generally used as a crucial component of polypharmacy in Traditional Chinese Medicine for disease treatments. Several experimental studies have recently documented that ethanolic extract of ZO (EEZO) is involed in many biologic activations including regulation of immunologic function, hypolipemic, antioxidation and antitumor. The present studies were designed to provide information regarding the effect of EEZO on mice with HepA tumor and ascites by assessing its survival time, immunologic function, apoptosis, against tumorous growth and antimutation.Seventy Kunming mice were randomly divided into 7 groups:control, HepA tumor, HepA tumor treated with high and low doses of EEZO, HepA ascites and HepA ascites treated with high and low doses of EEZO. Animals were subcutaneously and intraperitoneal injected with suspending liquid of HepA tumorous cells for the models of HepA turner and HepA ascites, respectively. A range dose from 10 (low) to 40 mg/kg (high) of EEZO was intragastrically administrated the mouse who has been successfully suffered HepA turner and HepA ascites for 12 d. After EEZO treatment, the varations of survival days, the inhibitory rate of tumor and immunologic functions including rhymus index, spleen index, percentage of phagocytosis, rate of a-ANAE+, level of hymolysin (IgM), LOH were respectively evaluated and compared to mice with HepA tumor or ascites. Apoptosis index of tumorous cells and loss of Fhit gene were respectively measured by TUNEL methods and allic special PCR.The results demonstrated that mice with HapA tumor and ascites treated by EEZO remarkably prolonged survival time (p<0.05). When mice with HapA tumor and ascites were dosed at EEZO 40 mg/kg, their survival time was prolonged to 50～60% compared with mice untreated. The administration of EEZO to mice with HapA tumor and ascites siginificantly proved their immunologic function such as increase in thymus and spleen index, percentage of phagoctosis, rate of a-ANAE+ and level of IgM; and significantly inhibited the tumorous growth. The inhibitory rates of tumor were 32.4% following EEZO 40 mg/kg administration. EEZO dose-dependently increased apoptosis index of mice with HapA tumor. When EEZO dosed at 40 and 10 mg/kg to mice with HapA tumor induced an increase in rate of Fhit gene loss by 40% and by 50% compared with mice with HapA tumor (70%) suggesting EEZO antimutation.Conclusion, treatments of EEZO to mice with HapA tumor and ascites induce a prolongation in survival time, an increase in immunologic function and apoptosis, and against tumorus growth and antimutation.