Objective To research the quantity, distribution and attribution of endogenous neural cell division and regeneration after spinal cord injury (SCI) in adult rats. And observe the expression of Notch-1 after SCI in rats. Methods 30 SD female rats were randomly divided into SCI and control groups. The normal control group (group A, n=5) and the spinal cord injury group(group B, n=25). The spinal cord injury were made at T8 level by Allen's method in group B. Double immunostaining were application to explore the quantity, distribution and attribution of newly BrdU-labeled mitotic dividing cells and investigate the expression of Notch-1 after SCI in 1,3,7, 14, and 28days post operatively in each group. Results Behavioral observation:after spinal cord injury, the experimental group rats were hindlimb weakness, slow walking, abnormal gait, motor dysfunction,and so on at different time. The HE staining observation:Structural integrity of the spinal cord and gray matter clear boundaries in control group. Disorganization and hydropic degeneration after spinal cord injury in experimental group. Immunohistochemistry staining: The expression of Notch-1 is not significant in control group, however, Notch-1 positive cells expression in every SCI group. We found that Notch-1 positive cell on the first day after SCI. The level of Notch-1 protein in NSCs increased significantly, peaked at 3 days and remained at high levels until 14 days after SCI. The expression of BrdU:A large quantity of BrdU-labeled cells appeared in the vicinity of the spinal cord lesion. The number was more than control group. Immunostaining of the injured T8 segment displayed a large quantity of BrdU-labeled cells after injury seven days by BrdU stereological cell number. The relation between Notch-1 and BrdU.Conclusion The level of BrdU protein was closely correlated with the level of Notch-1 protein and SCI can promote the proliferation of ENSCs in rats, which is correlated with the activation of Notch signaling pathway.
|