| Objective:This study was aimed to investigate the effects of cobalt protoporphyrin (CoPP) on hyperexpression of heme oxygenase-1(HO-1) and secretion of IL-10 in bone marrow-derived mesenchymal stem cells (BMMSC).Methods:The BMMSC were isolated from rats and culture,then were randomly divided into four groups, and were induced in culture medium with CoPP of 0,25,50, and 75mmol/L respectively. The expressions of CD34, CD45, CD44 and CD71 in BMMSC were detected by flow cytometry; the osteoblast and adipocyte induction of BMMSC were detected by alizarin red stain and oil red stain; the proliferation status was assayed by MTT method; the HO-1 expression in test group was detected by RT-PCR; the optimal concentration of CoPP for induction of HO-1 expression was screened.The ELISA kit was used to detect the IL-10. TGFβ1and IFN-γlevels in each groups.Results:The results showed that the expressions of CD34, CD45 in BMMSC were negative while the expressions of CD44 and CD71 in BMMSC were positive. After induced by osteoblast and adiopocyte inductor, BMMSC were positive for alizarin red staining and oil red staining. Various concentrations of CoPP had no significant impact on cell proliferation.When the CoPP concentration of 50mmol/Lcould induce strong expression of HO-1, expression level in test group was significantly higher than that in control group. After induced BMMSC IL-10 secretion was higher than that in control group, TGFβ1 and IFN-γsecretion did not change.Conclusion:It is concluded that CoPP did not have the ability to promote cell proliferation,50mmol/L is the optimum concentration for up-regulation of CoPP-induced BMMSC HO-1 expression, the up-regulation of HO-1 expression can increased IL-10 secretion significantly. |
