| Background: To explore the immune inflammatory response after bone marrow mesenchymal stem cells (BMSCs) transplantation in myocardial infarction rabbit. Acute myocardial infarction (AMI) is often accompanied by a strong immune inflammatory response. Recent studies have shown that BMSCs often have immunomodulation role and delay ventricular remodeling after myocardial infarction.Objective: To study the influence of transplanting autologous BMSCs on inflammatory response in heart dysfunction rabbit models, and to investigate the mechanism of transplantation of BMSCs for reducing the inflammatory response and improving myocardial remodeling; and to evaluate the treatment effectiveness on heart dysfunction after myocardial infarction by means of BMSCs transplantation.Method: Thirty-four male New Zealand white rabbits were randomly divided into three groups(sham group, heart dysfunction after AMI models group, BMSCs group).1 The rabbits models of AMI were established by means of ligated left anterior descending artery (LAD) via thoracotomy.2 BMSCs were isolated by means of density gradient centrifugation and cultured by adherence method and symbolized by DAPI.3 Autologous BMSCs were transplanted into the surrounding area of myocardial infarction to secondary to thoracic injection of autologous BMSCs in fourteen days after AMI.4 Echocardiography were taken at preoperative and seven days postoperative and twenty eight days after BMSCs transplant respectively.5 inflammatory cytokines expression: serum concentration of IL-6, TNF-α, CRP and MMP-9 by ELISA were taken at preoperative and seven days postoperative and twenty eight days after BMSCs transplant respectively.6 Myocardial NF-кB IL-6 TNF-αMMP-9 were detected by way of real time RT-PCR at twenty eight days after BMSCs transplant.Result:1 BMSCs separated and cultured in vitro: We can found the white BM-MNC layer between separating medium and blood plasma after density gradient centrifugation. The colony mixed together within eleven to fourteen days. When eighty to ninety percent colony had been mixed together, we taken digestion and passage.2 The establishment of heart dysfunction rabbits models after AMI. twenty eight days later, the twenty-four survival rabbits among the thirty-four acute myocardial infarction models were as follows, eight rabbits survival in sham group and eight rabbits survival in both AMI and BMSCs groups. The levels of LVEDD, LVEF and FS had no significant deviation preopration. Seven day after myocardial infarction the LVEF in BMSCs groups and AMI groups were significantly lower than those in sham groups(52.6±4.0 51.6±4.5 68.3±1.4, F=54.235, P<0.001),There were no significant deviation among BMSCs groups and AMI groups.3 The effects of autologous BMSCs on Cardiac function after AMI. At the end of twenty-eight days the ribbit's LVEDD in sham and BMSCs groups were obviously smaller(13.9±1.0, 14.7±1.4, 16.7±1.1, F=11.97, P<0.001),and the LVEF and FS were higher(EF:70.5±3.6, 67.0±5.8, 57.1±5.5,F=15.05,P<0.001;FS:34.5±3.1, 31.0±5.9, 25.5±4.4, F=7.02, P=0.005)than those in AMI groups.4 Inflammatory cytokines expression: The levels of serum IL-6, TNF-α, CRP and MMP-9 had no significant deviation preopration. Seven day after myocardial infarction the levels of serum IL-6, TNF-α, CRP and MMP-9 in sham groups were significantly lower than those in BMSCs groups and AMI groups (IL-6: 255.6±22.9, 373.4±123.7, 377.3±125.1,F=3.64, P=0.04;TNF-α: 161.5±17.3, 227.8±62.8, 227.9±63.6, F=4.25, P=0.02; CRP: 6.33±0.34, 10.18±3.71, 10.18±3.72, F=4.28, P=0.02; MMP-9: 62.6±11.2, 90.8±26.8, 89.7±28.8, F=3.65, P=0.04); There were no significant deviation among BMSCs groups and AMI groups.At the end of twenty-eight days, The levels of serum IL-6, TNF-α, CRP and MMP-9 in sham and BMSCs groups were obviously smaller than those in AMI groups(IL-6: 245.6±20.4, 227.5±21.3, 304.9±27.5,F=24.19, P <0.01; TNF-α: 160.3±16.0, 145.2±12.5, 197.5±13.7, F=28.91, P<0.01 ; CRP: 5.87±0.49 6.41±0.78 7.81±0.69,F=17.93, P<0.01;MMP-9: 63.0±9.5 58.9±4.2 80.7±7.4,F= 19.91, P<0.01).5 In marginal zone, in sham groups, BMSCs groups NF-κB, TNF-α, IL-6, MMP-9 expression were decreased than those in AMI groups(NF-кB:0.09±0.06, 0.59±0.47, 1.33±0.52, F=18.64, P<0.001;TNF-α: 1.00±0.43, 1.87±0.78, 3.70±1.64, F=13.06, P<0.001; IL-6: 1.12±0.66, 3.81±1.94, 6.03±1.85, F=18.86, P<0.001; MMP-9:1.64±0.67, 2.47±1.78, 5.53±2.33, F=11.04, P=0.001).6 The LVEF had a negative correlation with inflammatory cytokines and nuclear factor-кB expression.Conclusion:1 BMSCs transplantation can reduce the inflammatory response and nuclear factor-кB expression.2 BMSCs transplantation can retard left ventricular remodeling and improve cardiac function by reduce the inflammatory response.3 We obtained the BMSCs by means of gdensity gradient centrifugation and adherence, at the mean time we Successfully use DAPI as labeling marker after 5-azacytidine Induced.
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