The Influence Of Rosuvastation On Pdgf-induced Arterial Smooth Muscle Cells Phenotype Transformation

Background: Coronary artery disease is refers to coronary atherosc- lerotic ascular antrum stricture or obstruction, and coronary artery functional change cause myocardial ischemia anoxia or necrosis and cause heart attacks. The vascular smooth muscle cells(VSMC) are the main cell components of coronary atherosclerotic plaque, phenotype transformation of vascular smooth muscle cells is the key beginning step of the proliferaion and migration of vascular smooth muscle cells in coronary atherosclerotic heart disease with or without stent restenosis (ISR). The statins, 3-Hydroxy-3-Methylg-lutaryl-coenzyme A (HMG CoA) reductase inhibitors, are the most classical and effective cholesterol-lowering drugs. Many studies show that the statins have series of effects on anti-inflammation, anti-atherosclerosis, anti-proliferative, anti-oxidant stress, antithrombotic, the inhibition of angiogenesis and elevated level of plasma adiponectin to protect endothelial from damage. However, it is not yet reported whether the Statins can inhibit the phenotype transformation of vascular smooth muscle to prevent atherosclerosis.Objective: Our studies aim to observe the effect of rosuvastatin on preventing atherosclerosis by inhibiting the phenotype transformation of human umbilical arterial smooth muscle cells in vitro.Methods: 1.The original generation culture VSMC and identifycati- on. 2.10nmol/mL PDGF induction VSMC. 3. 1μmmol/l and 10μmmol/l rosuvastatin intervention VSMC. 4. The expression ofα-actin and osteop- ontin were performed by immunohistochemical Method.Results: 1 The expression ofα-SMA in is significantly decreased in PDGF induction group compared with control group(P<0.05). Rosuvastatin promote the up-regulation ofα-SMA relative to PDGF induction group. The expression levels ofα-SMA in 1and 10μmmol/l Rosuvastatin intervention groups are higher of PDGF induction group respectively, but both are lower than that in control group. There are significantly difference among them (p <0.05). 2 The expression of OPN in is significantly decreased in PDGF induction group compared with control group(P<0.05).Rosuvastatin promote the down-regulation of OPN relative to PDGF induction group. The expression levels of OPN in 1and 10μmmol/l Rosuvastatin intervention groups are lower of PDGF induction group respectively, but both are higher than that in control group. There are significantly difference among them (p <0.05).Conclusions: 1 PDGF promotes the transformation of VSMC pheno -type from contractile to synthesize phenotype. 2 The expression ofα-ac tin was significantly decreased, while the expression of osteop-ontin was significantly increased during the process of phenotype transformation. 3 high dose and low dose Rosuvastatin may inhibit the transformation of VSMC phenotype from contractile to synthesize phenotype with the association of elevated level ofα-actin and decreased level of osteopontin. And high dose is much more effective...