The Cytotoxicity And Genotoxicity Of Fine Particulate From Cooking Oil Fumes In Fetal Lung Type Ⅱ-like Epithelium Cells

Objective To investigate the cytotoxicity oxidative damage effects of oil fumes, formed when peanut oil is heated, on fetal lung type II-like epithelium cells(AECⅡ).Method The fine particulate was collected by PM2.5 vacuum pump, The fine particulate was extracted with acetone , the fine particulate were diluted to 200 mg/ml with dimethylsulfoxide (DMSO) at last. The rat mothers(18d) were killed and decapitation of the fetuses, fetal lung type II-like epithelium cells(AECⅡ) were isolated from Fetal lungs and identified. The AECⅡcells were treated with different concentrations of PM2.5, DMSO control for 12h,24 h and 48h. MTT assays were applied for testing AECⅡcell activity in vitro, glutathione (GSH), superoxide dismutase(SOD)and malondialdehyde(MDA) in Supernatant were determined to evaluate the cytotoxicity and oxidative damage effects of PM2.5 on AECⅡcells. Result The different concentration of PM2.5 can produced evidently cytotoxicity in a dose-and time-dependent response manner. PM2.5 exposure induced lipid peroxidation in a concentration and time-dependent manner. Statistically significant increases of MDA concentration were attained in AECII 24h and 48h after their exposure to PM2.5 at their 50g/ml (p<0.05), as compared with control cells . Statistically significant increases of MDA concentration were attained in AECII 48h after their exposure to PM2.5 at their 12.5 and 25g/ml (p<0.05), versus 12h and 24h. Statistically significant increases of MDA concentration were attained in AECII 24h and 48h after their exposure to PM2.5 at their 50g/ml (p<0.05), versus 12h .There were decreases of SOD activity in AECII 24 h and 48h after their exposure to PM2.5 at their 50g/ml (p<0.05), versus control cells. Statistically significant decreases of SOD activity were attained in AECII 48h after their exposure to PM2.5 at their 50g/ml (p<0.05), versus12h. There were decreases of GSH activity in AECII 48h after their exposure to PM2.5 at their 50g/ml (p<0.05), versus control cells. No statistically significant decreases of GSH activity were attained in AECII24h and 48h after their exposure to PM2.5 at their12.5, 25 and 50g/ml (p<0.05) in a time-dependent manner, versus 12h. Conclusion The fine particulate can produced evidently cytotoxicity in a dose-and time-dependent response manner. Objective To investigate the genotoxicity f oil fumes, formed when peanut oil is heated(PM2.5), on fetal lung type II-like epithelium cells(AECⅡ). Method The AECⅡcells were treated with 12.5,25 and 50ug/ml of PM2.5 for 12h,24 h and 48h. The comet assay was used to detect the Olive Tail Moment, Tail Moment, Tail Length and Tail intensity.The content of PAH- DNA adducts was detected by ELISA. Result Olive Tail Moment, Tail Moment, Tail Length and Tail intensity showed a significant increase with 50μg/ml, 25μg/ml and 12.5μg/ml treatment of PM2.5 compared to control. Sharp increase (P < 0.001) was observed in Olive Tail Moment, Tail Moment, Tail Length and Tail intensity in high dose groups compared to control and low dose group. There was a clear positive dose-dependent relationship between the results and the doses of PM2.5 (Table 1). Increase (P<0.001) was observed in Olive Tail Moment, Tail Moment, Tail Length and Tail intensity in 24h groups compared to 6h and 12h group. Significant difference (P<0.001) was found in Olive Tail Moment, Tail Moment, Tail Length and Tail intensity of 12h group compared to 6h group. Increase (P < 0.05) was observed in PAH-DNA adduct in high dose groups compared to control and low dose group.Conclusion The fine particulate can produced evidently genotoxicity in a dose-and time-dependent response manner.