Matrine Inflence On The Expression Of MRNA Level Of NF-κB,p53, DcR3 In Hepatocellular Carcinoma

Objective:To investigate the expression level of NF-κB(p65/RelA),p53,DcR3 in hepatocellular carcinoma cell line HepG2 which be treated with matrine and to explore the relationship among these factor and the mechanisms of HepG2 resist to matrine.Method:HepG2 cell were stimulated by different concentrations of matrine (0g/L, 0.25g/L, 0.5 g/L, 1.0 g/L, 1.5g/L). The HepG2 cell survival rates were evaluated by MTT assy. Cultured HepG2 cells were implanted in 6 well planted and divided into six groups: a control group, 4 matrine group,a LY294002 group (40μmol/L). Hoechst staining was used to detect apoptosis morphological change after Matrine treatment. The expression of NF-κB,p53,DcR3 mRNA level were measured using real-time polymerase chain reaction(RT-qPCR) in each group.Result:Matrine obviously inhibited the growth of HepG2 cells in a dose- and time- dependent manner. Hoechst staining showed apoptosis was induced after HepG2 cells were incubated with matrine only for 12h. The increase of NF-κB level and the decrease of p53 level were accompanied by the increase of the concentration of matrine. The mRNA level of NF-κB was correlated negatively with p53, but matrine did not affect the mRNA level of DcR3. In LY294002 group, NF-κB,p53,DcR3 mRNA level significantly higher than control level (p<0.001).Conclusion:Matrine could induce apoptosis, and at the same time induce activation of NF-κB in HepG2 cell. This maybe the mechanisms of Hepatocelluar carcinoma resist to matrine. The regulatory role of the matrine in NF-κB activity appears to be cell type-specific.