Study On Plasma Levels Of Lipids, Lipid Peroxidation And Inflammatory Factors In Gout Patients

Objective:The aim of the present study was to explore the plasma levels of lipids, lipid peroxidation and inflammatory factors in gout patients, then to investigate the possible mechanisms about high incidence of atherosclerotic diseases in patients with gout.Methods:Collect and record clinical data of 41 patients with gout (28 in acute episode phase; 13 in intermittent phase); 29 healthy volunteers from medical examination center as control group. The plasma levels of triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), uric acid (UA) were measured by enzymatic method, levels of apoprotein A (apoA), apoprotein B (apoB), lipoprotein(a)[LP(a)] and high sensitive C-reactive protein (hs-CRP) were measured by immunoturbidimetry; Levels of oxidized low density lipoprotein (ox-LDL), interleukin-1β(IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α(TNF-α) and transforming growth factor-β(TGF-β) were measured by enzyme linked immunosorbent assay (ELISA); Levels of malondialdehyde (MDA) and superoxide dismutase (SOD) enyzme activities were measured by colorimetric method; All experimental data about plasma levels of lipids, lipid peroxidation, and inflammatory factors were recorded and analyzed by statistical software, the relationships between some representative indexes were analyzed.Results:1) Compared with control group, levels of TC, LDL-C, and Lp(a) were significantly higher in patients with gout (P<0.05); The HDL-C level was significantly lower in acute episode phase gout than intermittent phase gout and control group (P<0.05). 2) The levels of ox-LDL and MDA were significantly higher in patients with gout compared to control group (P<0.05), the level of SOD in patients with gout was lower than control group (P<0.05); the level of ox-LDL in acute phase gout was significantly higher than intermittent phase gout (P<0.05). 3) The plasma levels of hs-CRP, IL-6 and TNF-αin patients with acute phase gout were significantly higher than intermittent phase gout and control group (P<0.05); Plasma level of TGF-βin acute phase gout was significantly lower than intermittent phase gout and control group (P<0.05). 4) The level of HDL-C was negative correlated with hs-CRP level in acute phase gout (r=-0.412, P=0.029). Plasma level of ox-LDL was positively correlated with MDA (r=0.318, P=0.043), but negatively correlated with SOD activities (r=-0.357, P=0.022) in two groups of gout. The level of ox-LDL were low-grade positively correlated with hs-CRP concentration (r=0.325,P=0.091) in acute phase gout.Conclusion:1) Plasma atherogenic serum lipid profile, which was associated with development and progress of atherosclerosis, was changed in gout patients. 2) Decreased activity of SOD and increased levels of lipid peroxidation can indicate that the oxidation-antioxidant system is defect in patients with gout, and they are susceptible to atherosclerosis. 3) Dyslipidemia and enhance of lipid peroxidation in acute phase gout were possibly associated with inflammation and oxidative stress.