Effects Of Nonylphenol On Brain Gene Expression Profiles Of F1 Generation

Objective: To investigate the adverse effects of nonylphenol(NP) on the nervous system function of F1 after gestation exposure and to explore whether the nonylphenol could affect the brain gene and protein expression of theF1 male rats.Methods: Pregnant rats were exposed to NP at doses of 0 and 200mg/kg body weights, respectively from pregnant days 7 to after pregnancy to 2 days post partum. The 2 days brain tissue of the F1 progeny of male rats were taken, mRNA were extracted. The RQ-PCR method and Western blotting were used to detect the expression of ubiquitin-specific protease 8 protein and AW141129, BE096678, BE111925, J02585, NM-021842 abnormal gene expression, and analysis of possible mechanisms.Results: The ubiquitin-specific protease 8 (USP8 / UBPy) gene and protein expression decreased; ATP-binding cassette transporter (ATP-binding cassette,ABCA) downregulated; nucleolar small nuclear ribonucleoprotein (MP4, U3 small nucleolar ribonucleoprotein, Imp4) upregulated-Endosα; ulfine protein Ensa (endosulfine alpha) downregulated; stearoyl coenzyme A dehydrogenase (stearyl-CoA desaturase, SCD) upregulated.Conclusion: The analysis on differences of gene expression and protein found that NP can be generated through various channels on various aspects of the body, which is Main to the nervous system function and development, interferes with endocrine system function, and affects steroid hormone synthesis and metabolism.