| Objective To investigate the effect of sodium-hydrogen exchanger 1 (NHE-1) on the occurrence and development of cardiovascular diseases and the role of NHE-1 in cardiac hypertrophy and chronic heart failure.Methods Sixty male Sprague-Ddwley (SD) rats were randomly and equally divided into 4 groups:Myocardial hypertrophy model were established by subcutaneous injection of isoproterenol (ISO), while control group 1 received an equivalent volume of 0.9% saline alone; Chronic heart failure group were injected of adriamycin (ADR) into the animals' abdomens at a dose of 2.5mg/kg once weekly for 6 weeks, while control group 2 received an equivalent volume of 0.9% saline alone. Left ventricular end diastolic diameter (LVEDD), left ventricular end systolic diameter (LVESD), interventricular septum thickness (IVST), fractional shortening (FS) and left ventricular ejection fraction (LVEF) were assessed by echocardiogram. The body weight (BW), ventricular weight (VW), left ventricular weight (LVW), ventricular weight index (VWI) and left ventricular weight index (LVWI) were determinated. The pathomorphological features of the myocardium were observed by electronmicroscope. Western blotting was used to detect NHE-1 protein content in myocardium. The expression of NHE-1 mRNA was determinated by RT-PCR. Plasma levels of NHE-1 were measured by Elisa.Results1 Significant difference was found between hypertrophy group and control group 1. IVST and LVPWT were larger in hypertrophy group than in control group 1 (P<0.01)2 Compared with those in control group 1, VW,LVW,VWI and LVWI in hypertrophy group were higher (P<0.01) 3 The myocardial and plasma levels of NHE-1 in hypertrophy group were higher than those in control group 1 (P<0.01). The content of NHE-1 mRNA in hypertrophy group was higher than that in control group 1 (P<0.01)4 Compared with those in control group 2, the degree of LVEF,FS,IVST and LVPWT in heart failure group were reduced (P<0.05). LVEDD and LVESD were enlarged (P<0.05)5 VWI and LVWI in heart failure group were increased than those in control group 2 (P<0.01)6 The myocardial and plasma levels of NHE-1 in heart failure group were higher than those in control group 2 (P<0.01). The content of NHE-1 mRNA in heart failure group was higher than that in control group 2 (P<0.01)7 The myocardial and plasma levels of NHE-1 and content of NHE-1 mRNA in hypertrophy group were positively associated with IVST (P<0.01)8 The content of NHE-1 in heart failure group were positively associated with LVEF (P<0.01). The plasma levels of NHE-1 were negatively associated with LVEF (P<0.01)9 The myocardial and plasma levels of NHE-1 and content of NHE-1 mRNA in heart failure group were higher than those in hypertrophy group (P<0.01)Conclusion Upregulation of NHE-1 is involved in the progress of cardiac hypertrophy and chronic heart failure.
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