The Expression Of 4EBP1 And Apoptosis Induced By CVB3 Infection In HeLa Cells

Objective:Coxsackie virus B3 (CVB3) was employed to infect the Hela cells.The influence of the Hela cell morphology and apoptosis were observed and the changes of the 4EBP1 expression were investigated too.Methods:CVB3 was employed to infect the Hela cells.The changes of cell morphological,and starved cell apoptosis with FITC-Annexin V /PI, the expression of 4EBP1 checked with RT-PCR and Real time-PCR were investigated.SPSS17.0 statistical software was used for statistical analysis, the P<0.05 was considered to be statistically significant.Results:1. After infected CVB3, the Hela cells turned to round and dark, floating like vacuolar or gathering like bunches samples under the light microscope.2. After CVB3 infected, Hela cells triggered apoptosis. The virus groups of Hela apoptosis rate were significantly higher than that in control groups (P<0.05), and the number of the apoptotic cell increased along with time.3. The expressions of 4EBP1 were checked with RT-PCR and Real-time PCR were found in both control and virus groups. The 4EBP1 expressions in the virus infected groups were decreased than that in the control groups (P<0.05), and the 4EBP1 expression in viral infected groups decreased with time extension.4. In the starved cells model, the expression of 4EBP1 checked with RT-PCR and Real-time PCR were found in both control and virus infected groups. The 4EBP1 expressions in the virus infected groups were decreased than that in the control groups (P<0.05), and the 4EBP1 expression in viral infected groups decreased with time extension.5. Compared with the starved groups, the 4EBP1 expression decreased in the control groups (P<0.05), suggesting that nutritional factors may affect the expression of 4EBP1.Conclusions: 1. Apoptosis were found in Hela cells infected with CVB3 and the apoptotic cells increased with time extension, which suggested that apoptosis may participate in the pathogenesis of CVB3 infection.2. The 4EBP1 expressed in normal or starved Hela cells, the expression declined after infected with CVB3, and deceased with the time extension, which suggested that 4EBP1 may contribute to the cardiomyocyte apoptosis mechanism.3. The expression of 4EBP1 may be affected by nutritional factors.