Experimental Study On Inflammatory Factors In Serum Of Rats With Rheumatoid Arthritis Treated By PengCao Removing Bi-syndrome Decoction

Objective:To observe the mechanism of PengCao Removing Bi-syndrome Decoction(PCRBD) on Rheumatoid Arthritis(RA) and the level of Inflammatory factors IL-1β,TNF-αarid PGE2 in serum of rats with Rheumatoid Arthritis treated by the decoction.Methods:Randomed out 10 rats as blank group, the remaining 50 rats for replication collagen inductive arthritis (CIA) model, divided them into Chinese high, medium, low group, tripterygium glycosides(TG) group and model group, keeped the TG group as the positive control group, observed 6 weeks, evaluated the inhibition of PengCao Removing Bi-syndrome Decoction on the joint swelling of the double back foot, the joint pain index of the double back foot, the weight gaining and the IL-1,TNF-αand PGE2 in serum.Result:Compared with model group, the joint swelling of the double back foot(P<0.01, P＜0.05), and the joint pain index of the double back foot (P＜0.01)of the rats in the treatment groups are significantly restrained; and the rate of the weight gaining improved significantly treated by the Chinese medium and high dose group (P＜0.01, P＜0.05); the level of IL-1β,TNF-αand PGE2 in serum of rats are much better than the model group(P<0.01); the inhibitory effect of Chinese medium dose group on the level of IL-1β,TNF-αis better than the TG group(P<0.05),the high dose group reduced the level of PGE2 better than the others.Conclusion:The usage of PCRBD could restrained the joint swelling of the right back foot and the joint pain index of the double back foot of the rats significantly. The mechanism of relieving the symptoms of rats with RA maybe related to reducing the level of proinflammatory cytokines IL-1β, TNF-αand PGE2 in serum, and the decoction is a pure Chinese herbal compound worthy of further research and development.