The Effect Of Apoptosis Of L-Gossypol On Human Fibrosarcoma HT1080 Cells And Its Underlying Mechanism

Objective:To investigate the anticancer activities of L-Gossypol on human fibrosarcoma HT1080 cell proliferation and the induction of the molecular mechanisms of tumor cell apoptosis. Methods:In this study, using human fibrosarcoma cell line HT1080 cells in vitro, observing the effect of L-gossypol on cell growth. The assay of MTT on HT1080 cell is to measure the proliferation; Hoechst33258 and the electron microscopy after using L-gossypol is to observe the morphological changes of apoptosis in HT1080 cells; flow cytometry assay is to measure the early apoptosis rate; colorimetry reflect the activity of caspase-9,3 changes before and after using L-gossypol; Western blot measure the levels of Bcl-2, Bax protein after using L-gossypol.Results:L-gossypol inhibit the proliferation of HT1080 human fibrosarcoma cells, and this inhibition depends on the working time and dose, the effect of L-gossypol is increasing by the increasing of the time and dose. HT1080 cells change the morphologic after using L-gossypol, Hoechst33258 reflect the high chromatin staining after using L-gossypol, electron microscopy show the typical morphological changes of apoptosis after using L-gossypol.The flow cytometry detect the effect of inducing early apoptosis on HT1080 cells by L-gossypol. In the process of L-gossypol inducing apoptosis in HT1080 cells, there are changing of Cacspase-9 and 3 activation. Anti-apoptotic protein Bcl-2 expression is decreasing and the expression of Bax is increasing depend on the increasing of concentration of L-gossypol.Conclusion:L-gossypol can inhibit the proliferation of HT1080 human fibrosarcoma cells and promote their apoptosis. L-gossypol may has the effect of inducing of apoptosis by increasing the caspase 9, caspase-3 activation, decreasing Bcl-2 protein expression, increasing expression of Bax protein in the regulation of play at the molecular level the role of apoptosis intumor cells.