Expression And Significance Of GRIM-19 And Its Target Gene Product STAT3 In Gastric Carcinoma

ObjectiveTo detect the expressions of GRIM-19 and its target gene product STAT3 in human gastric carcinoma(GC)tissues and explore their effects and possible mechanism during the occurrence and development of gastric carcinoma.MethodsApplying reverse transcriptase-polymerase chain reaction (RT-PCR) to detect the mRNA expression of GRIM-19 and STAT3 genes in 48 specimens of gastric carcinoma(GC)and evaluating the difference in various tissues. The protein expressions of GRIM-19 and STAT3 genes were detected by immunohistochemistry dyeing and western blotting, and the correlations of the expressions of gene GRIM-19 and STAT3 with various clinicopathologic characteristics of gastric carcinoma were analyzed statistically.ResultsThere were expressions of GRIM-19 and STAT3 genes in GC tissues, tissues surrounding carcinoma and normal tissues. The mRNA and protein levels of GRIM-19 were obviously lower in GC tissues as well as tissues surrounding carcinoma than those in normal tissues (P<0.01). Furthermore, the expression of GRIM-19 protein was correlated to cell differentiation and clinical stage of GC (P<0.01). In contrast, the mRNA and protein levels of STAT3 were higher in GC tissues as well as tissues surrounding carcinoma than those in normal tissues (P <0.01), and the expression of STAT3 protein was correlated to cell differentiation, lymphatic metastasis and clinical stage of gastric cancer (P<0.01). The protein expressions of GRIM-19 and STAT3 genes were not correlated to gender and types of tissues(P>0.05). Significantly negative correlation was found between the expression of GRIM-19 and STAT3 in GC tissues.ConclusionsThe low expression of GRIM-19 and the high expression of STAT3 co-exist in gastric carcinoma, and may be related to malignant transformation and abnormal proliferation of cells, which facilitates the development of gastric carcinoma.