| Objective: Clinical observation on hyperplasia of mammary glands of human disease, breast cancer, breast cancer Bcl-2 of apoptosis gene in precancerous lesions and Bax expressions, experimental study on the method of warming Yang Jie on treatment of hyperplasia of mammary glands in rats and combat apoptosis gene (Bcl-2) and apoptosis genes (Bax) effect, promote the study of cell apoptosis mechanism of hyperplasia of mammary glands treated by Chinese herbs.Methods: Clinical study on selected human precancerous lesions of breast hyperplasia and breast cancer, wax blocks of breast cancer specimens, immunohistochemical observation of Bcl-2 and expression of Bax in the pathological type. Experimental study on 100 Vister randomly divided into 5 groups of female rats, medial muscle injections of estradiol benzoate on its hind legs 0.5mg/kg/d, 25 days in a row, and then intramuscular progesterone 4mg/kg/d, 5 days in a row. Modeling successful rat (80) randomly divided into four groups: the control group, Chinese medicines, increased milk Ning group, treated with tamoxifen groups, each group of 20. Preparation of rat mammary gland, methods such as through HE staining, IMMUNOHISTOCHEMISTRY, observation of breast tissue morphology, and cell apoptosis resistant gene (Bcl-2) and apoptosis genes (Bax) expression.Results: (1) Bcl-2 in breast hyperplasia disease, and breast cancer Qian lesions and the breast cancer of positive expression rate (%) respectively for 95.2±1.32, and 90.4±3.02, and 77.65±4.09, expression has obvious differences (p<0.05); 64.1±3.02, and Bax in breast hyperplasia disease, and breast cancer Qian lesions and the breast cancer of positive expression rate (%) respectively for 76.75±3.51, and 64.1±3.02, and 38.95± 2.87, expression has obvious differences (p<0.05). (2) large rats blank group, and controlled group, and milk block Xiao soup group, and milk increased Ning group, and three benzene oxygen amine group breast organization form scoring respectively for 1±0.00, and 3.38±0.39, and 1.55±0.22, and 2.1±0.19, and 1.33±1.43, controlled group and blank group comparison, has obvious differences (P<0.01), description made die success; milk block Xiao Soup Group and controlled group, and milk increased Ning group, and three benzene oxygen amine group comparison, are has obvious differences (P<0.01). (3) large rats blank group, and controlled group, and milk increased Ning group, and milk block Xiao soup group, and three benzene oxygen amine group Bcl-2 expression rate respectively for 96.3±1.08, and 92.25±2.69, and 92.7±2.03, and 96.4±1.64, and 93.25±2.02,Bax of expression rate (%) respectively for 75.6±0.99, and 73.15±2.92, and 72.35±2.03, and 74.9±1.29, and 72±2.22, milk block Xiao Soup Group and controlled group, and milk increased Ning group Bcl-2, and Bax of expression comparison, are has obvious differences (P<0.01), milk block Xiao Soup Group and three benzene oxygen amine group Bcl-2, and Bax of expression comparison, has differences (P<0.05).Conclusion: Expression in precancerous lesions of breast cancer in clinical Bcl-2 achieve maximum, hyperplasia of mammary glands in reducing disease, breast cancer respectively; Expression of Bax as precancerous lesions of breast hyperplasia-decrease-increase of breast cancer lesions. By control groups and blank group comparison of pathological morphology and Bcl-2, Bax, with obvious differences, explains animal experiments combined estrogen and progesterone-induced rat mammary gland hyperplasia model modeling success; the application of hyperplasia of mammary glands in rats treated by warming Yang Jie, can reduce of mammary gland hyperplasia lesion on the morphological level, adjustable Bcl-2, at the molecular level expression of Bax. |
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