The Comparison Of Different Cycloxygenase Inhibitors' Effect On Ovarian Hyperstimulation Syndrome-model Rats

Objective:To evaluate whether cycloxygenase inhibitors can prevent and treat ovarian hyperstimulation syndrome or not. To compare effects of different kinds of cyloxygenase inhibitors, aspirin and celecoxib have different effects on OHSS or not.Methods:32 SPF-grade Wistar famale rats were equally grouped into 4: A:contral group, contral ovarian hyperstimulation; B:OHSS group, large dose gonadotropin ovarian hyperstimulation; C:celecoxib group, celecoxib tablets were taken before large dose gonadotropin ovarian hyperstimulation; D:aspirin group, aspirin tablets were taken before large dose gonadotropin ovarian hyperstimulation. The successful of animal model and the effects of aspirin and celecoxib were determined and compared by several methods such as weighing ovaries, determination of estradiol level in blood-serum and reverse transcription polymerase chain reaction.Results:The mean weight of ovaries in OHSS group is higher than other groups (P<0.05), and weights in other 3 groups have no differences. Serum E2 level is similar to that in OHSS group, celecoxib group and aspirin group with no great difference. But Serum E2 level in control group is significantly lower(P<0.05). RT-PCR electrophoresis-agarose gel result and gray-scale-analysis show VEGF's expression is higher in OHSS group than in others.Conclusion:Cycloxygenase inhibitors can prevent and treat ovarian hyperstimulation syndrome in animal models. Neither non-steroidal antiinflammatory drugs such as aspirin nor cycloxygenase-2 such as celecoxib, they both have the same effectiveness on preventing OHSS.