The Inhibitory Effects Of Bml-111, A Lipoxins Analog, On Pulmonary Metastasis In Hepatoma-bearing Mice

OBJECTIVES: To observe the pulmonary metastasis models of hepatoma-bearing mice induced by hepatoma cell line HepG2 and H22 cell transplantation in vivo, Compare the differences between xenotransplantation and homotransplantation, intraperitoneal and subcutaneous transplantation in two tumor metastasis mice groups, and introduce a method to establish a pulmonary metastasis mice models. To further research the effect of BML-111 in tumor microenvironment by morphological study.METHODS: We studied two mice models of pulmonary metastasis in vivo, human hepatoma cell line HepG2 and mouse hepatoma cell line H22 cells were cultrued in vitro, then applied hepatocellular carcinoma cells subcutaneous transplantation method to prepare pulmonary metastasis mice models, and observed whether the subcutaneous tumor is established, the initial time, tumor size and hepatoma cell proliferation condition in the lung tissue morphologically. Our experiment was consisted by two parts: PartⅠ, Research of two mice models of pulmonary metastasis on human hepatoma cell line HepG2 and mouse hepatoma cell line H22 cell transplantation in vivo. PartⅡ, The effects of BML-111 on pulmonary metastasis in hepatoma-bearing mice.RESULTS: PartⅠ, we didn't found palpable subcutaneous induration in HepG2 cell xenotransplantation group, but we detect palpable subcutaneous induration in H22 cell homotransplantation group. PartⅡ, the weight of the H22 + BML-111 homotransplantation group grow steadily in the first 7±1 days, and palpable subcutaneous induration was found in the first 11.5 days. The subcutaneous induration emerged later and its volume was significantly smaller than any other group (P <0.05).CONCLUSIONS: PartⅠ,The H22 cell homotransplantation mice model was simple, easy, reproducible and short cycle. PartⅡ, BML-111 inhibited the growth of solid subcutaneous tumors and pulmonary metastasis.