Preliminary Metabolomics Study Of Breast Cancer Based UPLC-Q/TOF And ~1 HNMR

Objective:~1HNMR and UPLC-Q/TOF based metabolic profiling was used to investigate the differences of serum metabolic patterns between patients with Breast cancer and healthycontrols to find tentative diagnostic biomarkers.Based on the findings above,we tried to describe the metabolite changes related to the continuous phases ofradiotherapy.It will provide a novel technological platform for the mechanism study of Breast cancer.Methods:1.Patients:16 newly diagnosed patients with Breast cancer who match the selection conditions served as Breast cancer group.11 healthy persons served as control group.2.Sample collection:Serum samples were collected from 11 persons in the control group through venous blood.Serum samples of 16 patients in the Breast cancer group were collected before chemotherapy and two weeks after chemotherapy.3.NMR analysis of serum samples:The NMR technique based metabonomic method was established,and the serum was introduced to NMR.Applying the NMR metheod,the serum samples from the health and Breast cancer were collected and detected by the machine. The serum endogenous metabolites were identified.4. Eatabilished the approach with Ultra-performance-Liquid Chromatography- ElectroSpray Ionization-quatrupole-Time Of Flight mass spectrometry (UPLC-ESI-Q-TOF)for metabonomic study,and introduced the method to the collecded serum to judge the state of disease.5. Data analysis:Then the data was imported SIMCA-P+ software for denoising. Through a variety of analysis of statistical analysis method of analysis produced massive data,analyzing process including: through the normalization, correction 80% rules, data set and data zoom in a set of data pretreatment method; Through theanalysis of data: principal by principal component analysis (PCA), slant component analysis, the least squares discriminant analysis (discriminant squares - order extra transportation charges nothing, PLS - DA) two methods of the samples classification; According to the model of variable importance factor (VIP value) and non-parameter test results and z value screening potential markers.Results:1. Eatabilished the approach with NMR for metabonomic study, some specific serum endogenous metabolites changes in the metabolic composition of serum samples from Breast cancer,there are lactate,valine, N-acetylgly-coproteins, glutamine,phosphocholine/choline, a-glucose/B-glucose,tyrosine,histidine,LDL/VLDL.2. Eatabilished the approach with for metabonomic study.Examination of the metabonomic data obtained by Ultra-performance-Liquid Chromatography -ElectroSpray Ionization-quatrupole-Time Of Flight mass spectrometry (UPLC-ESI-Q-TOF) ,the acquired metabonomic data was analyzed by multivariate analysis.Significant difference in endogenous metabolite profilea was observed in the control and patients with chemtheropy before and after.Conclusions:The relusts demonstrated that metabonomics is a potential and powerful tool for characterization of the metabolic perturbation under different physiopathologic status,and also indicated that the metabonomic analysis is promising to provide an integrative criterion to evaluate the severity of the Breast cancer and the prognosis.