Expressions Of HTERT MRNA And Bmi-1 In Breast Cancer And Their Significance

Objective Breast cancer is one kind of severely impair feminine physical and moral integrity common malignant tumor,Its morbidity is situated the feminine tumor continuously in the global scope the first place, and its disease incidence rate also by every year 2% speed increasing. The whole world has 1,200,000 women to contract breast cancer every year, 500,000 women die of breast cancer. Although our country is not breast cancer succeeding in the imperial civil service examinations country, but the yearly average rate of rise outdoes the succeeding in the imperial civil service examinations country 1~2 percentage points actually, by every year 3% speed increasing. Organizes Bmi-1 and the hTERT-mRNA expression situation through the examination breast cancer, discussion cancer gene Bmi-1 and hTERT-mRNA organize at in breast cancer the expression its and with the clinical pathology characteristic relations, appraises the two at in breast cancer occurrence development function and the significance, as well as between the two relevance.Methods (1) immunity group: Uses the immunity group SP law to examine 49 example breast cancers to organize the Bmi-1 expression, analyzes Bmi-1 in breast cancer expression and with the pathology characteristic relations; (2) RT-PCR method: Uses the RT-PCR law to examine 49 example breast cancers to organize the hTERT-mRNA expression situation, analyzes hTERT-mRNA in breast cancer expression and with the pathology characteristic relations. Counts the tool to use the SPSSl3.0 software package, the statistical method uses the card side examination and the Spearman rank correlation analysis, take P=0.05 as the significance examination standard. Results (1) The immunity histochemistry result showed in 49 example breast cancer specimen the Bmi-1 strong masculine gender rate is 83.67%(41/49), nearby the corresponding cancer organizes Bmi-1 exceptionally to express rate is 18.37% (9/49),There was a significant difference between the tumor tissues and normal breast tissues(P<0.05).The RT-PCR result showed hTERT-mRNA organizes at the cancer the masculine gender rate is 79.59%(39/49), nearby the corresponding cancer organizes the masculine gender rate 10.20%(5/49) there was a significant difference between the cancer samples and adjacent non- cancerous samples (P<0.05).(2)The abnormal expression of Bmi-1 in breast cancer with lymph node metastasis was 96.42%(27/28),organizes the masculine expression rate in non-lymph node shift breast cancer is 66.67%(14/21).After statistics analysis demonstration, the two comparison has statistics significance (P<0.05).The abnormal expression of Bmi-1 increased with the TNM stage increased.The expression of Bmi-1 level was not correlated with ER,PR,VEGF,c-erbB2, tumor size, age,or tumor pathological types(P>0.05).The abnormal expression of hTERT-mRNA in breast cancer with lymph node metastasis was 84.44%(24/29).In breast cancer without lymph node metastasis,the abnormal expression rate of hTERT-mRNA was 51.61%(16/31).There was a significant difference between them(P<0.05).The Bmi-1 exceptionally expression elevates along with tumor TNM by stages markup, its expression level with estrogen acceptor (ER), progestogen acceptor (PR), VEGF, c-erbB2, the tumor size, the age, the tumor pathology type has nothing to do with. hTERT-mRNA in has breast cancer which the lymph node shifts to organize the masculine expression rate is 92.86%(26/28), But its expression level with estrogen acceptor (ER), progestogen acceptor (PR), VEGF, c-erbB2, the tumor size, the age, the tumor pathology type has nothing to do with.(3)Relationship between Bmi-1 and hTERT-mRNA expression in breast cancer:In the Bmi-1 masculine specimen the hTERT-mRNA high expression, occupies 97.43%(38/39). After the Spearman rank correlation analysis demonstrated the two assume obviously is being connected (r=0.379, P<0.001).Conclusion :1. Bmi-1 organizes at breast cancer to express the obvious ascension, its expression level and the tumor organization's lymph node shift and TNM are by stages close are connected, with estrogen acceptor (ER), progestogen acceptor (PR), VEGF, c-erbB2, the tumor size, the age, and the tumor pathology type does not have the relevance.2. hTERT-mRNA organizes at breast cancer to express the obvious markup, its expression level and the tumor lymph node shift and TNM have the relevance by stages, with estrogen acceptor (ER), progestogen acceptor (PR), VEGF, c-erbB2, the tumor size, the age, and the tumor pathology type does not have the relevance.3. Bmi-1 and hTERT-mRNA both organize at breast cancer the expression present correlation.4. Bmi-1 and the hTERT-mRNA exceptionally expression possibly develops at breast cancer occurrence and makes a sneak attack shifts the vital role, examines both to be possible jointly to take breast cancer biology behavior and so on early time diagnosis, judgment prognosis and shift important norms for reference.