The Experimental Study On Hypoxia Induced Plexin B1 Expression In Tumor Cells

Objective: The hypoxic tumor microenvironment induced angiogenesis, but the specific mechanism was unclear. In this experiment, A549 human lung cancer cells, HepG2 human liver cancer cells were studied, to investigate the expression of Plexin B1, which was relevant with tumor angiogenesis, under different hypoxia condition.Methods: Cells cultured in hypoxia conditions and normoxia condition, and then analyzed the difference expression of Plexin B1 with different hypoxia time within the tumor cells by immunofluorescence and Real-time PCR assay.Results: Immunofluorescence showed that the signal of Plexin B1 located on cellular membrane and Plexin B1 expression up-regulated in hypoxic condition and reached peak until hypoxic keeping on 18 hours. The result of Real-time PCR is same with immunofluorescence.Conclusion: Hypoxic can induce Plexin B1 expression, and the expression increases as the persistence of hypoxic, which cued that Plexin B1 up-regulation may particpate the progression of hypoxia-mediated angiogenesis.