| Objective Previous study showed that salidroside can eliminate fatigue obviously. We select swimming rats as experimental model. Intervention groups were given different doses of salidroside respectively for 12d continuously. Through swimming tests, the Energy Substances and mitochondria function in Skeletal Muscle were measured to observe its effect on anti-fatigue.Methods Use the method of testing swimming time to investigate anti-fatigue effect of salidroside. Use the method of kits and HPLC to test the content of energy substances in skeletal muscle cells of periph fatigue rats. Using the method of spectrophotometer to test the absorbance of mitochondria in skeletal muscle cells of periph fatigue rats can reflect the open degree of MPTP indirectly. Use the method of kits and spectrophotometer to test the activity of mitochondria electron transport chain complex III and IV in skeletal muscle of periph fatigue rats. Use the method of fluorence microplate reader and spectrophotometer to test the content of ROS and MDA and the activity of Mn-SOD.Results The result showed that swimming time of three dose salidroside intervention groups(50mg/kg,100mg/kg,200mg/kg) were lengthened by 20%,37.7%,62.6% than the control group, and the trend was more obvious as the dose increased. It's indicated that salidrosid can lengthen swimming time of rats and increase the capability of exercise. The result showed that the content of glycogen and ATP of three dose salidroside intervention groups were both increased more obviously than the control group, and the trend was more obvious as the dose increased. It's indicated that salidrosid can increase the content of energy substances in skeletal muscle cells of periph fatigue rats. The content of glycogen of three dose salidroside intervention groups were increased to normal lever at 12h, and continued to increase. It's showed that salidroside can promote the increase of glycogen. The content of ATP of three dose salidroside interventionn groups were increased from 6-24h, and decreased from 24-48h. It's showed that salidroside can inhibit the decrease of ATP and promote the increase of ATP at the convalescent period of fatigue. There was the most significant effect of high dose salidroside intervention group. The result showed that the open degree of MPTP from three dose salidroside intervention groups were decreased more obviously than the control group, and the trend was more obvious as the dose increased. It's indicated that salidrosid can decrease the swelling of mitochondria to protect its membrane structure. Using the method of CLSM to observe the FI of mitochondria△ψm in skeletal muscle cells of periph fatigue rats, the result showed that the FI of mitochondria△ψm from three dose salidroside intervention groups were increased more obviously than the control group, and the trend was more obvious as the dose increased. It's indicated that salidrosid can inhibit the decrease of△ψm caused by the open of MPTP to protect its membrane structure. As the convalescent period of fatigue lengthened, the absorbance and FI of mitochondria both increased continuely, the open of MPTP was inhibited, and the loss of△ψm was controlled. There was the most significant effect of high dose salidroside intervention group. The result showed that the activity of mitochondria electron transport chain complex III and IV were inhibited by periph fatigue, and the respiratory function of mitochondria was decreased to influence the generation of energy substances. The activity of mitochondria electron transport chain complex III and IV in skeletal muscle from three dose salidroside intervention groups were increased more obviously than the control group, and the trend was more obvious as the dose increased. It's indicated that salidrosid can recovery the decrease of mitochondria electron transport chain complex III and IV activity. There was the most significant effect of high dose salidroside intervention group. The result showed that the content of ROS and MDA from three dose salidroside intervention groups were decreased and the activity of Mn-SOD was increased more obviously than the control group, and the trend was more obvious as the dose increased. It's indicated that salidrosid can increase the activity of Mn-SOD to decrease the generation of ROS and MDA. The content of ROS and MDA were increased from 6-12h, and decrease from 12-48h. The activity of Mn-SOD was decreased from 6-12h, and increase from 12-48h. It's indicated that salidrosid can weaken the oxidative stress damage caused by ROS and MDA through increasing the antioxidation capability of mitochondria. There was the most significant effect of high dose salidroside intervention group.Conclusions Salidroside can lengthen swimming time of rats and has significate effect on anti-fatigue. Through increasing the activity of electron transport chain complex III and IV in skeletal muscle mitochondria, salidroside can recovery the capability of respiratory function, increase the capability of ATP regeneration and control the degradation of glycogen to achieve the effect of increasing the content of energy substances in skeletal muscle cells. Salidroside also can decrease the swelling of mitochondria and inhibit the decrease of mitochondria△ψm to protect its membrane structure Through assisting the antioxidation system to increasing the activity of Mn-SOD in skeletal muscle mitochondria, salidroside can eliminate the generation of ROS and MDA and recovery the oxidative stress damage caused by periph fatigue to achieve the effect of anti-fatigue.
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