A Study Of Nested Quadriplex PCR With Common Primers For DYS-714/723/650/713 Loci And Application Of Forensic Science

Objective The subject is to establish a Y-STR loci nested quadriplex PCR amplification system with a pair of common primers. Four Y-STR loci were selected, which showed high heterozygosity among 20 Y-STR loci investigated by our laboratory previously. The amplification system has been evaluated for forensic science casework.Method Four Y-STR loci were chosen for four Y-STR loci multiplex system. These quadriplex PCR products shared the same sequence at two ends with specific primers added by a common tail at 5'ends. By this way the first round PCR products could be further amplified by a pair of common primers to avoid unballanced amplification due to different loci. The PCR products were visulized by silver stain.Results A set of Y-STR quadriplex amplification system consisting of DYS714, DYS723, DYS650, DYS713 loci was constructed. The PCR results showed more consistent than that of traditional multiplex amplification system. The five hundreds picogram templtes could be detected in our quadriplex system. By our quadriplex system there showed the same genotype with templates from different tissue at same individul, and no cross-reactions were observed between human being and animals. The samples contaminated by women biological stain did not interfere with genotyping.Conclusion We successfully set up a new Y-STR quadriplex with a common tail PCR products, which could be amplified by a pair of common primers. The approach could be used in forensic caseworks.