Limb Ischemia And Reperfusion On Spinal Cord Neurons C-fos Expression And The Intervention Of Flurbiprofen

Objective:To observe the condition of limb ischemia and reperfusion in rat neurons after spinal c-fosexpression, and to explore limb ischemia and reperfusion on spinal cord neurons c-fosexpression and flurbiprofen in rats.Methods:Adult male SD rats were 78, were randomly divided into four groups:①sham operation group (S group, n= 24), only to be anesthetized and dissected femoral vessels and femoral nerve, do not do other treatments.②limb ischemia 4h and reperfusion group (IR group, n= 24).③morphine+limb ischemia 4h and reperfusion group (M+IR group, n=24), established before ischemia model in rats with intrathecal injection of morphine 10μg/100μl. More than 3 groups according to reperfusion time (1h,4h,8h,12h) was divided into 4 phase, 6 rats in each phase.④flurbiprofen in the intervention group (F group, n= 6), the M+IR group basis, respectively, before injection of morphine and 10min after reperfusion in rat tail vein injection of flurbiprofen injection 2mg·kg-1, the 4h of reperfusion time of one phase. End of the experiment, rats were killed by cardiac perfusion, lumbar spinal cord taken (L1-5) by immunohistochemical method (ABC method) to detect the rat spinal cord Fos protein positive neurons in the expression and distribution.Results:Compared with S, IR, M+IR group at each time after reperfusion in rat spinal cord fos immunoreactive neurons (FLIN) were significantly increased number (P<0.05). Reperfusion 1h,4h,8h FLIN when the number of IR group than M+IR group (P<0.05),12h number of the two groups showed no significant difference FLIN. IR group phase in four hours when the FLIN 1h largest number of distribution to the spinal cord mainly shallow,4h,8h,12h, fos protein expression in order to reduce, but the distribution is broader, before and after each layer and the angle around the central canal have expressed. M+IR group 4h peak when the number of FLIN,8h began to weaken after the angular distribution around the main layers and the central canal, no significant changes in distribution. F group with the M+IR group when compared FLIN 4h significantly reduced the number (P<0.05).Conclusion:Ischemia-reperfusion induced spinal cord corresponding segments of fos immunoreactive neurons (FLIN) significantly increased the number of reperfusion, the increase in the initial FLIN ischemia and reperfusion may be mainly generated by the pain caused, as the reperfusion time and the pain stimulation reduced ischemia-reperfusion injury may be the main reason for the late FLIN increase. We conclude that the corresponding segments of spinal cord neurons may be involved in the pathogenesis of IR/I limbs, especially the central canal of the spinal cord and peripheral neurons may have a more important role, flurbiprofen injection possible in spinal cord after limb IR/I has a protective effect, this effect may be related to inhibition of the c-fosexpression.