Studies On The Changes Of Adiponectin, Resistin And Adipocyte Fatty Acid-binding Protein In Abdominal Obesity And Their Clinical Significance

Background 2005 International Diabetes Federation (IDF) issued a global consensus definition of Metabolic Syndrome(MS)that abdominal obesity is a prerequisite to diagnosis of metabolic syndrome , emphasizing the importance of abdominal obesity in the metabolic disorder. Abdominal obesity is an independent risk factor of coronary heart disease, type 2 diabetes, cerebrovascular disease, hyperlipidemia. Because the fat in different parts have different degree of metabolic and endocrine, abdominal obesity may be due to intra-abdominal fat accumulation associated factors secreted quality and quantity of change, and trigger MS. Adiponectin(ADPN), Resistin(RETN) and Adipocyte fatty acid binding protein(A-FABP) are recently described adipocytokines which produced by adipose tissue. They have been recognized as a key regulator of obesity, insulin sensitivity, metabolic syndrome and atherosclerosis. The role and mechanism of circulation ADPN, RETN and A-FABP in preventing and treatment of obesity and MS are still to remain to research.Objective This study measured abdominal obesity and Metabolic Syndrome in serum and adipose tissue ADPN, RETN, A-FABP levels, to explore the abdominal obesity and MS patients ADPN, RETN, A-FABP and waist circumference, body mass index, blood glucose, and lipids and its clinical significance, in-depth study ADPN, RETN, A-FABP and their roles in the physiological mechanism, further clarify the obese, MS pathogenesis, preventing and treatment of obesity and MS for the lay a theoretical basis. Methods According to the International Diabetes Federation (International Diabetes Federation, IDF) of the MS diagnostic criteria for selection of 10 patients with abdominal obesity, MS12 patients in this study. Collection of objects on the history, physical examination and sacrificing blood and subcutaneous and omental adipose tissue. ELISA method to measure fasting serum ADPN, RETN, and A-FABP; using RT-PCR method for the determination of fat tissue ADPN, RETN, and A-FABP mRNA; simultaneous determination of blood glucose, blood lipid levels and other related indicators.Results1. MS group, abdominal obesity group than in control subjects of BMI, WC, WHR significantly different (all P <0.01); systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (ABPm) among the three groups no significant difference; TG in the control group, obesity group, MS group gradually increased (all P <0.01), abdominal obesity and MS patients than in the control group increased TC, HDL-C reduced only in the MS group (P <0.05); FPG increased only in the MS group (P <0.01).2. ADPN in the MS group, central obesity group than in the control group reduced (P <0.05), while the MS group than in obese group had no significant difference; RETN, A-FABP in the MS group increased (P <0.05), and the MS group than in obese group increased (P <0.05): the A-FABP in the MS group than in the control group and central obesity were higher (P <0.05).3. Omental and subcutaneous adipose tissue ADPN, RETN, A-FABP mRNA expression differences (P <0.05).4. Abdominal obesity group, MS group omental adipose tissue adiponectin mRNA expression were lower than the control group, the differences were statistically significant (P <0.05); each group of subcutaneous fat, the relative expression of adiponectin mRNA level of no significant difference.5. Abdominal obesity group, MS group omental adipose tissue expression of resistin mRNA levels were relatively higher, the differences were statistically significant (P <0.05); MS group relative subcutaneous fat resistin mRNA expression levels rose compared with the control group higher (P <0.05).6. MS, omental and subcutaneous adipose tissue adipocyte fatty acid binding protein mRNA relative expression levels than the control group, obese group were higher (P <0.05).7. Omental fat ADPN, RETN, A-FABP mRNA and BMI, WC, WHR correlated (P <0.05). Adipose tissue ADPN, RETN, A-FABP mRNA expression were correlated (all P <0.05).Conclusions1. Subcutaneous and omental adipose tissue adiponectin, resistin, and adipocyte fatty acid binding proteins are different, omental adipose tissue decreased expression of adiponectin, resistin level and adipocyte fatty acid binding proteins expression.2. Adiponectin, resistin, and adipocyte fatty acid binding protein expression and abdominal obesity are closely related to obesity with decreased expression of adiponectin, and resistin, and adipocyte fatty acid binding protein expression.3. Adiponectin, resistin, and adipocyte fatty acid binding protein exists interrelated, decreased expression of adiponectin, resistin and may be accompanied by adipocyte fatty acid binding protein expression.