BDNF, TAC Levels In Follicular Fluid Of Patients With PCOS, DOR And Their Effect On The Outcome Of In Vitro Fertilization And Embryo Transfer

Objective:To explore effect of brain-derived neurotrophic factor (BDNF), total antioxidant capacity (TAC) on polycystic ovary syndrome (PCOS), diminished ovarian reserve (DOR) and in vitro fertilization /embryo transfer(IVF-ET) outcome, through comparative analysis of BDNF, TAC levels in follicule fluid, clinical characteristics, outcomes of IVF-ET among women with PCOS, DOR and normal ovulatory function.Methods:96 infertile women under the age of 42 undergoing IVF-ET were sampling randomly, including 28 women with PCOS,34 women with DOR and 34 women with normal ovulatory function as the control group. GnRH agonist down-regulation long protocols were used in PCOS and control group, while GnRH agonist down-regulation short protocol was used in DOR group. In the morning of the day of oocyte retrieval, a little clear follicle fluid was acquired from each of the 96 patient. BDNF levels were detected by enzyme-linked immunosorbent assay and TAC levels were detected by colorimetry method. All patients whose embryos were finally transferred were grouped into pregnancy group and non-pregnacy group. Statistical analysis of all datas was performed with SPSS 13.0.Results:1. Compared with the control group, patients with PCOS had significantly higher levels of serum basal lutein hormone(LH), higher ratios of serum basal LH to follicle stimulating hormone(FSH), lower fertilization rate after IVF-ET. Compared with control group, patients with DOR had higher levels of serum basal FSH, higher ratios of serum basal FSH to LH, less good quality embryos, lower rate of good quality embryo.2. Patients with PCOS or DOR had significantly higher follicular-fluid BDNF and TAC levels, compared with controls.3. TAC level adversely correlated with good quality embryo rate.4. In the control group, patients who were pregnant after embryo transfer had more good quality embryos, higher cleavage rate, higher rate of good quality embryo than patients who were not pregnant. In PCOS group, pregnant women had higher fertilization rate, higher rate of good quality embryo than non-pregnat. In DOR group, pregnant women had higher rate of good quality embryo than non-pregnant. In control group, TAC levels were significantly elevated in non-pregnant patients, compared with pregnant, but this significant difference was not showed in PCOS or DOR group.Conclusion:1. BDNF, TAC are significantly elevated in the follicular fluid of paients with PCOS or DOR.2. Excess follicular fluid TAC influences good quality embryo rate, and is unfavourable for the pregnancy outcome of IVF-ET in patients with normal ovulatory function.