| Objective: Neonate has a high degree of susceptibility to pathogens. Sepsis is easily occur in newborn, which has a high mortality. Controlling sepsis is still a global problem currently. Inherent immunity has innate immune defense function, which forms gradually in germ-line development and evolution of the long-term. Macrophage Cell is an important member of body's natural immune system, whose functional status affects the prognosis of infection directly. CD163 is a membrane glycoprotein of macrophage,in the process of infectious inflammation, which could mediate the endocytosis of LPS and the release of anti-inflammatory cytokine, and have anti-inflammatory effects. On the other hand, CD163 could shed from the surface of macrophages into blood, become sCD163. sCD163 has the effect of anti-inflammatory and immunomodulation. In view of this, we established the model of E.coli septicemia of neonatal rats to analysis the variation of macrophage surface CD163 expression and plasma sCD163, in order to approaching the mechanism of CD163 in sepsis, and attemptting to look for a new possible marker for the diagnosis of sepsis.Methods: Applying 72 7-day-old Sprague-Dawley(SD) rats with Speeifie Pathogen Free(SPF),whose weight is from 15g to 20g, male or female is open. they were divided into two groups: control group(36 rats) and experiential group(36 rats). All the rats were randomly divided into different time points including 2,4,6,12,24,48 hours, each time point had 6 SD rats. In the experiential group we established sepsis model through peritoneal injection with Escherichia col(iE.coli). Control group was peritoneal injection with the same amount of normal sodium. After anesthetizing animals in target time points, we took blood from heart and obtained lungs and livers for analysis. We did immunohistochemical analysis of macrophage surface CD163 of lungs and livers, and carried out the ELISA detection of plasma sCD163.Results: 1. In the group of experimental group, the expression of macrophage surface CD163 from the rat's lung and liver stepped down gradually at every time point with the time prolonged; 2. In the second hour of the experiment, the expression of macrophage surface CD163 in lung and liver had little obviously difference between the experimental group and control group (p > 0.05 ); the distinction has appeared remarkable statistical significance since the forth hour of the experiment (p<0.01); 3. The level of sCD163 rised step by step at every time point in the experimental group. In the second hour,the level of sCD163 had no difference between the experimental group and control group(p>0.05 ); Comparing with the level of sCD163 between experimental group and control group, they had statistic meaning following the develpoment of experiment (p<0.01 ).Conclusion: 1. In the course of E.coli sepsis of neonatal rats, the expression of macrophage CD163 and plasma sCD163 significantly deviated comparing with control group, which prompted that CD163 has significant function in the development of sepsis process. 2. The level of macrophage surface CD163 in lung, liver and plasma sCD163 of neonatal rats declined with time-dependent in the progress of sepsis, which pointed that the defensive function of macrophage diminished and the body damage increased. 3. The level of neonatal rat plasma sCD163 upgraded with the development of sepsis, indicating that the body changed from pro-inflammatory response to anti-inflammatory state gradually. 4. The diversity of macrophage surface CD163 of neonatal rats immediately reflected the pathogenetic condition and prognosis of organism after sepsis. On the ground, we tried to look for a new possible marker for the diagnosis of sepsis. |
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