A Nest Case-control Study On The Association Between Genetic Polymorphism On Peroxisome Proliferator-activated Receptors And Hypertension

Objective1.To explore whether PPARαIntron 7G>C(refsnp4253778),PPARδc.2806C>G(refsnp9794),PPARγ681C/G(refsnp10865710)are associated with hypertension in community population of JiangSu province.2. To evaluate the association among genetic polymorphism and interaction between genetic polymorphism of PPARs and main hypertension environmental risk factors.MethodsThis study covered 352 hypertension patients and 468 normotensive controls. The variant of PPARαIntron 7G>C was determined by the restriction fragment length polymorphism PCR method and PPARδc.2806C>G,PPARγ681C/G were determined by the TaqMan probe real-time PCR method. Logistic regression was used to evaluate association of genotype or allele frequencies with hypertension and select environmental risk factors. Addictive model and multiple model were applied to assess the interactive effects between gene mutation and environmental factors on hypertension.Results1. Three gene polymorphisms were consistent with Hardy-Weinberg expectations in control groups.2. The CC homozygote of PPARδc.2806C>G in hypertension patients were higher than that of the control group and showing a statistically significant difference(P=0.023).Significant difference in genotype or allele frequencies of PPARγ681C/G was observed(P<0.001,P=0.003, respectively).After ajusted for covariates age,gender,smoking,drinking and hypertension family history, CG+CC genotype frequencies of PPARδc.2806C>G polymorphism and CG+GG genotype frequencies of 681C/G were responsible for the increased risks for hypertension (aOR=2.23(95%CI:1.06-4.69), aOR=1.61(95%CI:1.21-2.16),respectively).3. Data through logistic regression analysis showed that the risk of hypertension increased with age and income growth (trend test P<0.001 ).Low fiber but fatty diet, obesity and hypertension family history were independent risk factors(aOR=2.55(1.84-3.52),1.93(2.05-4.20), 1.71(1.23-2.29), respectively).4. Positive interaction between PPARαIntron 7G>C mutation and Low fiber but fatty diet was found, the aOR was 1.10(95%CI:1.02-1.19,P=0.016)in multiple model and interactive index S=4.00(1.36-11.79)in addictive model. No other interaction among PPARαIntron7G>C,PPARδc.2806C>G,PPARγ681C/G and between gene and envirenmental risk factors were observed.Conclusion1.Our result suggested that the PPARδc.2806C > G and PPARγ681C/G polymorphism were associated with hypertension in community population of JiangSu province. The C allele of PPARδc.2806C>G and G allele of PPARγ681C/G might be the risk factors for hypertension.2. No interactions on hypertension among PPARαIntron 7G>C,PPARδc.2806C>G,PPARγ681C/G have been found.3. Interactive effect between PPARαIntron 7G>C mutation and Low fiber but fatty diet both in addictive model and multiple model was observed.