Study Of Anti-hiv Drugs Molecularly Imprinted Polymers Applied To Solid Phase Extraction

In this research, molecular imprinting technique and solid-phase extraction were reviewed in detail. In addition the advances were in analytical methods of zidovudine (AZT) and lamivudine(3TC) in real samples were summarized. Using non-covalent bulk polymerization method, AZT molecularly imprinted polymers (MIP) and 3TC MIP were synthesized and packed into the SPE cartridges. The application of molecular imprinting solid-phase extraction(MI-SPE) in real samples was discussed. The main results of the studies are as follows:1. The best monomer was chosen by combining computer simulation and breakthrough experiment; the esterified product of 3TC(3TC-Es) was synthesized. Besides, Molecular imprinting was used to develop a method based on non-covalent interaction for the synthesis of AZT and 3TC-Es imprinted polymer. The molecular recognition characteristics of the imprinting polymers were evaluated by UV. This method provided basic evidence for synthesizing anti-HIV drugs molecularly imprinted polymers and analysising its mechanism.2. Using methacrylic acid as functional monomer, and trimethlolpropane trimethacrylate as cross linking agent, MIP specific for AZT was synthesized; in the same way, using methacrylic acid as functional monomer, ethylene glycoldi methacrylate as cross linking agent, a MIP specific for 3TC was synthesized using 3TC analog 3TC-Es as template, then were packed into SPE cartridges. By optimizing the loading, washing and eluting conditions, AZT, 3TC and their analogues were separated by the MI-SPE method successfully. It laid foundation for separation and enrichment of drugs in real samples. At the same time, the problem of"template leakage"was solved successfully by suppositional molecular imprinting technique.3. After cleaning off the interfering compounds, the content of anti-HIV in real samples was determined: It was found that MI-SPE-HPLC can be used for the determination of AZT and 3TC. The recoveries of AZT in serum were in the range of 73.8%~76.3%, and 3TC in serum were 69.9%~75.0%. This method is rapid, intuitive, also can be used to separate and enrich drugs of other biomedical sample.