| Objective:To study the protective effects and possible mechanism protective effects of Rapamyin on hepatic ischemia reperfusion injury in rats.Methods:The left-median liver lobe ischemia-reperfusion model was performed in rats.45 adult and health rats were divided randomly into the following 3 groups(15 rats each group):Group A, sham group; Group B:pretreated the ischemic reperfusion model with saline; Group C: pretreated the ischemic reperfusion model with Rapamyin (0.5mg/kg/d Rapamyin 5 days before operation). The group B and C were blocked the left-median liver lobe with a clamp for 90 minutes, and five rats in each groups were executed at 2h,6h and 24h postoperation. Blood samples were obtained to measure the levels of TNF-a,IL-1,ALT and AST. Liver tissues were collected at the same time to observe histologic findings, the stain of p-STAT3 was detected by immunohistochemistry and the level of TNF-a mRNA by RT-PCR.RESULT1.Compared with Group A, the serum levels of AST, ALT and IL-1 in group B and C were significantly improved; pathologic histology tests found out Group B and Group C's extent of disease and IRI were much more serious; The ratio of positive cells of STAT3 in Group B and Group C was higher by Immunohistochemisty. The level of TNF-a mRNA detected by RT-PCR was increased. (P<0.05)2. Compared with Group B, the serum levels of AST, ALT, IL-1 and TNF-αin group C were decreased; Pathologic histology findings in Group C were alleviated; The Scores of Congestion, Vacuolar degeneration, Cellular necrosis were lower; the expressions of p-STAT3 and levels of TNF-a mRNA in Group C were decreased. (P<0.05)CONCLUSION1. By inhibiting the STAT3 signal transduction pathway and following inflammation-related genes and protein expression, Rapamycin reduces the hepatic ischemia-reperfusion injury.2. Hepatic ischemia-reperfusion injury can activate the STAT3 signal transduction pathway, and the activation of STAT3 may be involved in the process of hepatic ischemia-reperfusion injury.
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