Effects Of Shuanghuanglian On The Changes Of L-type Calcium Channel Current By Aconitine Of Cultured Rat Ventricular Myocytes

Effects of shuanghuanglian on the changes of L-type calcium channel current by aconitine of cultured rat ventricular myocytesBackgroundThere are a variety of ion channels on the cardiac cell, such as INa, ICa,Ikl,Ikr and Iks, etc. each ion channel has its own specific features and characteristics. The various ion channels Coordination to maintain normal myocardial systolic and diastolic function. When some drug effects on these ion channels, may lead to arrhythmia. With the patch clamp technique to develop and mature, there are a large number of experimental studies the role of drug-induced arrhythmia mechanisms and the best role of antiarrhythmic targets.Cardiovascular system that is the main target organ damaged by Aconitine poisoning.which cause serious arrhythmia is the main reason of death. But there is no effective medicine treat the aconitine poisoning.In early work, through a large number of animal experiments and clinical observation showed that Shuanghuanglian treat aconitine poisoning have significantly effect.Shuanghuanglian can quickly save aconitine induced arrhythmias may be related to ion channels, in order to further observation the Shuanghuanglian on the detoxification mechanism of aconitine, in this experiment,using whole cell patch clamp technique to observe the effects of shuanghuanglian on the changes of L-type calcium channel current by aconitine of cultured rat ventricular myocytes.Objective1. To determine the effects of aconitine and shuanghuanglian on L-type calcium channels in the ventricular myocytes of the rats.2. To study the pharmacological mechanism of aconitine and Shuanghuanglian intervene after aconitine on the ion channels of myocardium. And the pharmacological mechanism Of Shuanghuanglian against arrhythmia caused by the aconitine.MethodsSingle wentricular myocytes of rats were obtained by primary culture method, Divided into normal group, aconitine group, and shuanghuanglian intervention group.The influence of aconitine and Shuanghuanglian intervene after aconitine on L-type calcium channels was recorded by the technique of whole-cell patch clamp.ResultsAt the test potential of+10mv, aconitine increased L-type calcium current from (-1104.00±252.3085) PA to (-3163.44±272.1169) PA (p < 0.05).Join Shuang huanglian, the Ica-L peak back to (-1512.57±488.0761)pA. Aconitine download the current-voltage curve, but activation potential, peak potential and the shape ofⅠ-Ⅴcurve, reversal potential unchanged. Aconitine and Shuanghuanglian on Ica-L activation curve does not significantly effectoin. Aconitine and Shuanghuanglian there half active potential (V0.5) respectively is (19.4207±2.9148) mv and (17.3374±2.4404) mv (P> 0.05).The steady activation was no statistically significant. The impact on the time effect curve, the rise time of aconitine is 87s or so, wash it away, the current also not recover, but Join Shuanghuanglian after, the curve decrease rapidly (56s) return to the status before administration.Conclusion1. Aconitine in rat ventricular myocytes can significantly enhance the Ica-L..2. Shuanghuanglian can markedly inhibit the increased of Ica-L by aconitine on ventricular myocytes.