Effect Of Corticotrophin-releasing Hormone On The Connexin-43 Phosphate And On Gap Junction Intercellular Communication In Human Cervical Smooth Muscle Cells

ObjectiveTo investigate the effect of human Corticotrophin-releasing Hormone (CRH) on the expression of Connexin-43(Cx43) phosphate in uterine smooth muscle and the function of cell gap junction intercellular communication (GJIC) in uterine smooth muscle.MethodPrimary culture human non-conceive uterine smooth muscle cells. With different concentrations of CRH intervention (0,5.85pm/l,58.5pm/l,585pm/l, 5850pm/l), adopt western blot testing Cx43 phosphate and Cx43 non-phosphate of protein expression in uterine smooth muscle. Use cell scratch to test cell gap junction intercellular communication (GJIC) opening status in uterine smooth muscle.Result1 Effect of corticotrophin-releasing hormone on the connexin-43 phosphate and connexin-43 non-phosphate in human cervical smooth muscle cells:In the uterine smooth muscle cells, the expression of Cx43 phosphate: 0.10±0.00,0.26±0.03,0.31±0.01,0.51±0.01,0.61±0.04,(P<0.01), and concentration-dependent trend existing; the expression of Cx43 non-phosphate:0.21±0.011,0.20±0.012,0.21±0.09,0.20±0.05,0.21±0.01, (P>0.05).2 Effect of corticotrophin-releasing hormone on the gap junction intercellular communication in human cervical smooth muscle cells:With different concentrations of CRH intervention, the transmission of cell layers: 0.83±0.40,2.17±0.40,3.67±0.528..33±0.52,9.33±0.55(P<0.01),and the time of it:8.33±0.52,7.77±0.52,5.33±0.82,1.67±0.52,1.17±0.41 (minutes) (P<0.01). ConclusionsIn the primary culture uterine smooth muscle cells, CRH can enhance the expression of Cx43 phosphate, and the gap junction intercellular communication (GJIC).