Expression Of FN And Study On The Inhibition Of Floxuridime In Human Choriocarcinoma

Objective(1)To detect the expression of FN (fibronectin) in different aggressive cells (JEG-3 and JAR) of choriocarcinoma, and investigate the relationships between the high expression of FN and the invasion and metastasis of choriocarcinoma.(2)To explore the inhibitory effect of FUDR (floxuridine) on the high metastasis-ability of human choriocarcinoma JEG-3 cell line in vitro, and to compare the efficacy and the expression of FN that of floxuridine with 5-FU.Methods(1)Transwell Chamber assay was performed to compare the effect of the JEG-3 and JAR cell lines in invasion capacity;(2) Expression of FN mRNA and protein level in the human choriocarcinoma cell lines(JEG-3 cells and JAR cells)were detected by real time-PCR and western blotting, and then compare their differences;(3)The human choriocarcinoma cells(JEG-3 cells and JAR cells)cultured in vitro were treated by FUDR and 5-FU with concentration of 0.05, 0.25,0.5,2.5,5ug/ml for period of 48h. MTT assay was used to determine the growth inhibition by FUDR and 5-FU on the JEG-3 cells,and calculate the relative survival rate.Western Blotting assay was used to detect the expression of FN by FUDR and 5-FU with concentration as 0.5ug/ml.Results(1)This exprement investigate that the invasion ability of JEG-3 cell line was stronger than that of JAR cell line(P<0.01);(2) In both of the two kinds of cells can detect the expression of FNmRNA,The expression of FN mRNA and protein level in JEG-3 cells were significantly stronger than that in JAR cells(P<0.001 and P<0.05);(3)FUDR and 5-FU can inhibit growth of JEG-3 cell and manner of the concentration-dependent, the inhibition ability of FUDR was significantly stronger than that of 5-FU with concentration as 5,2.5, 0.5ug/ml(P<0.05).The expression of FN protein level in JEG-3 cells were treated by FUDR with concentration as 0.5ug/ml significantly lower than that by 5-FU.Conclusion(1)The existence of FN gene in JEG-3 and JAR cell lines may play an important role in the metastasis and invasion of humanchoriocarcinoma.(2)FUDR can inhibit growth of the high-metastasis JEG-3 cell line,the inhibition ability of FUDR was significantly stronger than that of 5-FU in the certain concentration.FN protein level in JEG-3 cells were treated by FUDR with concentration as 0.5ug/ml significantly lower than that by 5-FU.