The Effects Of Advanced Glycation End Products On Profilin-1 And Asymmetric Dimethylarginine In Vascular Endothelia1 Cells

Objective:To study the effects of advanced glycation end products on profilin-1,ADMA and NO in the cultured human umbilical vein endothelial cells, so as to explore the possible mechanisms of endothelial injury caused by advanced glycation end products.Methods:Human umbilical vein endothelial cells were incubated with different concentrations of AGEs-BSA (50ng/ml,100ng/ml,200ng/ml) for various periods of time (6h,12h,18h,24h). The protein expression of profilin-1 in cells, the levels of ADMA and NO in the conditioned medium were determined. Simultaneously, control group and BSA group were prepared.Results:AGEs-BSA significantly upregulated the expression of profilin-1 in a concentration-and time-dependent manner, and BSA had no effect on the expression of profilin-1. AGEs-BSA significantly increased the level of ADMA in a concentration-and time-dependent manner, and BSA had no effect on the level of ADMA. AGEs-BSA(200ng/ml) significantly decreased the level of NO in the conditioned medium after 24h.Conclusion:AGEs(200ng/ml) upregulated the expression of profilin-1 and increased the level of ADMA, which implicated AGEs could induced vascular endothelial cells injury by means of upregulating the expression of profilin-1.