The Effects Of Hyperthermia Combined With Chemotherapy And Immunotherapy On Human Lung Cancer Cell Lines A549 In Vitro

BACKGROUND Lung cancer, which is harmful to human health, is one of common tumors. The morbidity and mortality rates are rising year by year. The therapeutic efficacy of advanced lung caner is poor. With the development of modern medicine, more and more lung cancer treatments are emerging, such as surgery, radiotherapy, chemotherapy, hyperthermia, biotherapy and so on. Combined therapy is more beneficial for patients, which is a combination of different treatments. Hypertherapy could inhibit tumor development, relieve symptoms and increase the susceptivity of other treatments. Cisplatin (DDP) and Docetaxel (TXT) are the commonest chemotherapy agents used to treat nonsmall-cell lung cancer. Sapylin is an immunologic agent, which could accommodate your body's defence system and kill tumor cells directly. Combined therapy including thermotherapy chemotherapy and immunotherapy is applyed in clinical practice confusedly. What's more, the therapeutic efficacy is not ideal. Therefore we must carry out a large number of fundamental and clinical experiments to consummate lung cancer treatments.This research studies the synergistic effect of combined use of DDP, TXT, Sapylin and hyperthermia on human lung adenocarcinoma cell line A549 in vitro.METHODS 1.There are four influencing factors including DDP, TXT, Sapylin and hyperthermia. Firstly, A549 cells were treated with different concentrations of DDP, TXT, Sapylin and different temperatures of hyperthermia singly. Secondly, every two influencing factors were used concurrently. Cell viability was measured at 24h and 48h respectively by means of MTT assay. The anticancer combined effects of hyperthermia and drugs were evaluated by Veleriote method. The anticancer combined effects of every two drugs were evaluated by coefficient of drug interaction (CDI);2. A549 cells were treated with Sapylin singly, DDP, TXT and hyperthermia separately or the combination of them. The cell apoptotic rates were examined by means of flow cytometry (FCM) at 48h.3. A549 cells were treated with Sapylin singly, DDP, TXT and hyperthermia separately or the combination of them. The morphologic change of control group and treatment group cells were observed by the optical microscope and the fluorescence microscope at 48h.RESULTS 1.The significantly proliferation inhibition effects were observed when using either hyperthermia or drugs alone (P<0.05) at 24h and 48h, demonstrating a temperature or dose–dependent manner.2. Hyperthermia can enhance the proliferation inhibition rates compared with chemotherapy agents alone (P<0.05); There is no statistical difference in the proliferation inhibition capability between hyperthermia singly and combined use with sapylin statistically (P>0.05).3. Significant synergistic effects were observed when DDP combined with TXT or sapylin (P<0.05), but not TXT combined with sapylin (P>0.05).4. The apoptotic rate of combined use of DDP2μg/ml, TXT1μg/ml at 42°C is higher than other groups (P<0.05). The results are coincident with morphologic observation.CONCLUSION Sapylin could inhibit A549 cells proliferation and induce apoptosis directly in vitro. Significant synergistic effects were observed when sapylin combined with DDP, but not with TXT or hyperthemia. Synergistic antitumor effects of combined use of DDP, TXT and hyperthermia on A549 cells were prior to other groups, including significant proliferation inhibition and apoptosis induction.