| Objective:To establish the post-status epilepticus model of temporal lobe epilepsy in rat that mimics human temporol lobe epilepsy, particularly the occurrence of spontaneous recurrent seizures(SRS), which was used to investigate the mechanisms of epilepsy or to test new antiepileptic compounds.Methods:Status epilepticus which was monitored 3 hours was induced by a 20 min stimulation of the amygdala(100ms train of 1ms,60Hz bipolar pulses,400μA,every 0.5s). Stimulated rats(n=21) were monitored with a video recording system for the 3-months follow-up period to record the appearance of SRS.Results:SRS(total number 181) were detected in 14 of the 21 animals (67%) after a latency period of 8 to 73 days (mean 43 days),including stage 3 seizure 10 (5.53%), stage 4 seizure 77(42.54%), stage 5 seizure 94(51.93%). Of all the 14 rats, the seizure number beyond 30 were observed in 3 animals, from 20 to 30 in one animal, fome 10 to 20 in 2 animals, between 5 and 10 in 2 animals, less than 5 in 6 animals. Seventy-three percent of SRS appeared during daytime (07:00-19:00)and seventy-four percent of secondarily generalized seizures occurred during daytime.Conclusion:This study has been successfully designed to develop a post-status epilepticus animal model induced by electrical stimulation of the amygdala, which has been laid the foundation for the further research of pathology, molecular biology and treatment of temporal lobe epilepsy. In addition, some indicators of our animal model are observed and researched, and are compared with other models of temporol lobe epilepsy.
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