The Expression And Significance Of Caspase-3, E-cadherin, And PTEN Protein In Hilar Cholangiocarcinoma

Objective To detect the expression of Caspase-3, E-cadherin, and PTEN protein in hilar cholangiocarcinoma and normal bile duct tissue, analyzing the correlation between three targets and their clinical significance. To investigate their role they will play in the occurrence and development of hilar cholangiocarcinoma and accumulate data for occurrence, invasion, metastasis and prognosis prediction of hilar cholangiocarcinoma.Methods We collected 50 patients'(27 males and 23 females) hiiar cholangiocarcinoma paraffin-embedded tissue samples with complete clinical follow-up data in Hepatobillary Surgery department of the affiliated hospital of Qingdao University Medical College from the year 2004 to 2008 At the same time, we selected the Organization of the normal common bile duct in 10 cases as controlled group. Immunohistochemical S-P method was used to detect the expression of Caspase-3, E-cadherin, and PTEN protein in the hilar cholangiocarcinoma and normal paraffin sections. At last, we applied SPSS 11.5 statistical software to analyze the experimental data.Results The low expression rates of Caspase-3, E-cadherin, and PTEN protein in hepatic portal cholangiocarcinoma tissue are respectively 70%, 68% and 58% and those in the normal bile duct tissues are 30%,20% and 10%. Compared the expression of Caspase-3, E-cadherinand PTEN protein in the Hilar cholangiocarcinoma tissues with that in normal common bile duct tissues, the difference is significant (P<0.05). The expression of Caspase-3, E-cadherin and PTEN protein in the Hilar cholangiocarcinoma tissues has no relation with patients' sex, age, preoperative serum bilirubin level, clinical classification and surgery (P> 0.05). The low expressions of all three are closely related to tumor invasion and metastasis (P<0.05). The expression of Caspase-3 and PTEN protein in the Hilar cholangiocarcinoma has no correlation (r=-0.165, P> 0.05); That of E-cadherin and Caspase-3 is positively correlated (r= 0.523, P<0.01); That of E-cadherin and PTEN protein is not related (r= 0.111, P> 0.05).Conclusion The expressions of Caspase-3, E-cadherin and PTEN protein in hilar cholangiocarcinoma tissue are low and the difference is significant, compared with normal bile duct tissues. It suggests that Caspase-3, E-cadherin protein and PTEN protein may be involved in the occurrence, development, invasion and metastasis of hilar cholangiocarcinoma and have relation with the patient's survival. The low expression rate of Caspase-3 and E-cadherin protein in hilar cholangiocarcinoma tissues has significantly positive correlation, and they may play a synergistic role in the invasion and metastasis in the hilar cholangiocarcinoma.