Study Of The Anti-atherosclerotic Effect Of Yincha Compound In Rabbits With Experimental Atherosclerosis

Objectives:To investigate the ability of anti-atherosclerosis of Yincha compound by observe changes the serum lipid levels, the area of plaque,the expression of nuclear factor kappaB and matrix metalloproteinase-2,and then to ascertain its mechanisms,and compare the effect of tea polyphenols and inko biloba extract so as to provide the therapeutic basis to material research and clinical coronary disease.Methods:Thirty five male New Zealand rabbits were randomly allocated into five equal groups:NC group;AS group;YCH group;YCL group;Sim group;TP group and GBE group.The NC group were fed with basis forage,the rest were fed with high-fat gorage.The NC group,AS group,YCH group,YCL group,Sim group,TP group and GBE group were independently given Yincha compound, Yincha compound, Simvastatin, tea polyphenols,ginko biloba extract 500mg/﹒kg-1﹒d-1,200mg﹒kg-1﹒d-1,5mg﹒kg-1﹒d-1,250mg﹒kg-1﹒d-1,250mg﹒kg-1﹒d-1 , They were fed for 10 weeks in all.Serum levels were examined before experiment and at the end of 10 week and AI were calculated. The histomorphology of the aorta was observed by HE stain and MASSON stain.The IT,MT,I/M and atherosclerotic area ratio were measured by image analysis software. The protein expression of NF-κB,MMP-2were detected by immunohistochemitry staining.The mRNA levels of MMP-2 were detected by RT-PCR.Results:At the end of 10 weeks, In all high cholesterol-fed rabbits,serum lipid level increased remarkly, The typical fatty streaks and early atheromatous Plaque on the aorta have been formed and the expression of NF-ΚB and MMP-2 in the lesion were inereased significantly. Compare to atherosclerosis group:①the thickeness of intima and media,and the area of atheromatous Plaque were lower in YCH group and YCL group,especially in YCH group(P<0.05);②the thickeness of intima and media,and the area of atheromatous Plaque were lower in TP group and GBE group,especially in TP group,and the comnination of both of them gain a best effect(P<0.05).Conclusions:Yincha compound prevent the development of atherosclerosis through lowering lipid level, oxidizing reaction,stabilizing plaque and inhibiting inflammatory reaction.