Association Study Between Single Nucleotide Polymorphisms On 8q24 With Prostate Cancer Risk In Chinese Han Population

BACKGROUND.Recently, independent genome-wide scans have identified multiple genetic variants at 8q24 to be associated with PCa risk. The rs1447295 was the first variant identified by two independent study groups,and it was shown to have the strongest association in European and African populations. This association was replicated in European Americans, African Americans, Japanese Americans, Native Hawaiians, Japanese,and Asian Indians. Gudmundsson et al identified a second variant rs16901979 at 8q24 associated with PCa risk. At about the same time, Haiman et al identified five previously unidentified risk variants at 8q24, of which rs6983561 showed the strongest association with PCa. Most of the association studies have been performed in American, African and European populations. We performed this study to determine whether these three risk variants at 8q24 were associated with PCa risk in Chinese han population.METHODS. We conducted a case-control study comprising of 120 prostate patients and 120 healthy controls in Chinese han population. Genotyping by direct sequencing was performed for rs16901979, rs6983561and rs1447295. Their association with disease stage, tumor grade, PSA level and disease aggressiveness was also determined.RESULTS.1. The allele C of rs6983561 was not associated with prostate cancer risk, odds ratio was 1.19(95% CI:0.81-1.76,P=0.367). The risk allele C was associated with prostate cancer risk in PCa patients of both stages (A+B) and stages (C+D),and in patients with aggressive disease, odds ratio was 3.54(95% CI:2.38-5.25; P=0.000), 1.60(95% CI;1.02-2.51; P=0.040),1.78 (95% CI:1.16-2.67;P=0.008) respectively.2. The allele A of rs 16901979 was not associated with prostate cancer risk,odds ratio was 1.13(95% CI:0.76-1.66,P=0.55).The allele A of rs16901979 was not associated with prostate cancer risk in PCa patients of all stages, all Gleason scores, all PSA level and all disease aggressiveness groups(P>0.05)3. The A allele of rs 1447295 was significantly associated with prostate cancer risk,odds ratio was 1.63(95% CI:1.02-2.63,P=0.042). The allele A of rs1447295 was associated with PCa risk in patients with PSA levels>20 ng/mL and in patients with aggressive disease,odds ratio was 1.63 (95%CI:1.00-2.66;P=0.033) and 1.63 (95% CI:1.00-2.67;P=0.049) respectively.CONCLUSION. The variants rs1447295 at 8q24 is associated with prostate cancer risk in Chinese han population. The A allele of rs1447295 carrying can increase the risk of prostate cancer.