The Application Of Confocal Laser Scanning Microscopy In The Diagnosis Of Epidermis Proliferative Diseases And The Evaluation Of Therapeutic Effect

Objective:1.The aim of the study was to test the applicability of CLSM to the diagnostic discrimination of Seborrheic keratosis and Plane warts.And their architecture aspects observed by Confocal laser scanning microscopy, and to correlate them with routine histopathology.2.Evaluate the therapeutic efficacy of narrow-band UVB phototherapy in combination with topical tazarotene.to plaque psoriasis.by confocal laser scanning microscopy.Method:1.Images of CLSM were contrasted with the histopathology to induce the criteria. Blinded retrospective analysis of the images from the lesions was carried out by the criteria to determine the specificity and sensitivity for diagnosis.2.Efficacy was assessed separately by means of a modified psoriasis severity index (PSI).The thickenesses of epidermis and amount of capillary were measured before and after the Phototherapy. Aimed to asess whether the addition of tazarotene to narrow-band UVB phototherapy more effective, faster clearing of psoriasis compared with NB-UVB monotherapyResults:1.There was a good correlations between the seborrheic keratosis and Plane warts dermoscopic patterns and findings of confocal images.Byχ2 test, multivariate discriminant analysis and binary logistic regression were performed for the identification of the significant features and for testing newly created diagnostic models. Seborrheic keratosis were mostly characterized by"Gyrus-like"structure,acanthosis,horn cysts.Plane warts were mostly characterized by"Flowers-like" Structure and vesicular cell. the sensitivity and specificity of confocal features for the diagnosis of seborrheic keratosis were 100% and 87.5%.the sensitivity and specificity of confocal features for the diagnosis of plane warts were 89.7% and 90.9%.2.Both treatment modalities notably reduced the PSI scores and the thickness with values being significantly lower in skin areas.But the addition of tazarotene to narrow-band UVB phototherapy more effective, faster clearing of psoriasis compared with NB-UVB monotherapy.Conclusion:1.there are high sensitivity and specificity of confocal features for the diagnosised of seborrheic keratosis and plane warts.2.The addition of tazarotene to narrow-band UVB phototherapy promotes more effective, faster clearing of psoriasis compared with NB-UVB monotherapy...