| Objectives:To set up UV-induced cataract model of SD rats and compare the effects of three kind of image acquisition on lens. Thereby provide correlated experimental basis for researching the formation, development, grades of cataract and prevention of cataract. Methods:1. three months-old first class rats (20subjects) were randomized to A or B group. Group A as experiment that put rats in the cage (20×40×20cm3) which was installed UVB lamp. The UVB lamp was 40cm from the rats. The rats was irradiated for 2 hours per day. Group B as control group that did nothing. Pick the rats'eye ball at different time:5-day,20-day,40-day, and remove corneal, and observe the degree of lens turbidity and the change of lens nucleus. Cut the anterior lens capsule roundly and pave it on the glass slide.Detect the apoptotic cells cells with TUNEL method in 20X high power field perimeter and observe the morphologic change of the lens epithelium cells.2. four other rats were selected to E grup for comparing the effects of three kind of image acquisition on lens. Results:1.Observe the lens with anterior eye segment image getting system:experimental group shows the SD rats'lens which receive UVB radiation 2 hours per day keep transparent at the 5th day. The cortex becoming feeble opacity and concentrate round anterior "Y"suture, peripheral cortex opacity as white lamellar anteriorly and featheriness toward ambitus, transparency and color change of the lens nucleus were not seen at days 20. The opacity is more obvious at anterior subcapsular cortex. white haze was seen at anterior peripheral cortex and deep cortex, and extend to ambitus at days 40. The superficial lamella cortex was adhered to anterior capsular more tightly than normal. And the 40-day's radiation shows the color change was not obvios. On control group, the lens keep transparent each time.2. TUNEL apoptosis detection:TdT-mediated X-dUTP nick end labeling reflect that anterior lens capsule epithelium had apoptosis after radiation at 5th day, the apoptosis cells present claybank.3. Morphologic change of cells:The change of shape and size for non-apoptosis lens epithelium cells were not obvious compared with control group at 5th day. The aging phenomenon on lens epithelium cells appeared at 40th day as lens fiber grew out of the epithelium cells.4. the image got by using anterior eye segment image getting system combined with lens pallets were clearer than other tow methods Conclusions:1. By using ultraviolet B radiation we set up UV-induced cataract model in SD rats successfully.2. Ultraviolet B was one of the factors that cause cataract.3. There was a positive correlation between the degree of opacity and radiation lasting time.4. Ultraviolet B radiation can cause anterior lens epithelium cells apoptosis in vivo.5. Three methods of anterior eye segment image getting system each has merits and demerits. Anterior eye segment image getting system combined with lens pallets can get ideal lens images and has obvious superiority by contrast with other tow methods in our experiment.
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