The Effects Of Berberine Hydrochloride On The Vascular Endothelzal Cells Injured By Glucose,Insulin And Low-Denslty Lipoprotein

Objiective:To investigate the effects of Berberine on the vascular Endothelzal cells Injured by Glucose,Insulin and oxidized low-Denslty Lipoprotein. Methods:We make Human umbilical vein endothelial cell line Huvec expose to fixed concentration of Glucose,Insulin and low-Denslty Lipoprotein (Glu40mmol/L,Ins20mU/L,ox-LDL 20mg/L) for 48 hours and investigate the effects of different concentration of Berberine on this injured vascular Endothelial cells.The cells in each group were cultured for additional 48 hours, then cell s viability was examined and the supernatants were used to determine the contents of endothelin-1 (ET-1), nitric oxide(NO), Inter leukin6(IL-6), Tumor Necrosis Factor (TNF-a),Super-oxide Dismutase(SOD). Results:1) the effect on cell proliferation rate:the proliferation rate of the model group decreased significantly compared with the normal group (P<0.01), it showes the success of model. Treatment groups and Captopril group could significantly improve the activity of endothelial cells and showes certain degree of dose-dependent. The treatment group and model group were statistically significant differences (P<0.01).2) the effect on NO content: the content of NO was 54.047±3.946μmol/L in normal group,and the model group was 9.881±2.082μmol/L. NO contents of the treatment groups were 73.724±4.404,67.958±2.573,64.370±4.342,57.013±3.721,55.176±2.410μmol/L.the NO content in Captopril group was 66.258±2.118μmol/L,compared with normal group,model group were significantly different (P<0.01), showed the model group was successful; the contents of NO in treatment group 1-3 and Captopril group were higher than normal group, difference between groups were statistically significant (P<0.01) and the effect of treatment group 1 was stronger than the captopril group (P<0.01); 3) the effect on ET-1 content:The Ber decreased the content of ET-1 and had dose-dependent. The content of ET-1 was 38.101±2.911ng/L in normal group whereas the model group was 60.240±3.058 ng/L. ET-lcontents of the treatment groups were 39.412±2.890,45.656±2.984,48.976±2.914,52.292±1.254.56.373±1.099ng/L. the ET-lcontent of Captopril group was 45.337±2.971ng/L, compared with normal group, model group was significantly different (P<0.01), showed the model was successful; compared with model group, treatment groups were statistically significant (P<0.01) and the effect of treatment group 1 was stronger than the captopril group(P<0.01); 4) the effect on SOD content:Compared with normal group, model group were significantly different (P<0.01), showed the model was successful; treatment groups did not achieve normal group level; There were significant difference between model group, treatment groups and Captopril group (P<0.01), and the effect of treatment group 1,2 was stronger than the captopril group (P<0.01); 5) the effect on IL-6 content:IL-6 contents of normal group were 36.840±3.578 pg/ml, the content of model group was 517.800±24.092pg/ ml, IL-6 contents of treatment group were 42.635±6.216,60.417±8.593,70.593±7.019,81.195±4.204,85.937±9.073 pg/ml. IL-6 contents of Captopril group was 70.069±9.740 pg/ml. there was significant difference between model group and treatment group(P<0.01), and the effect of treatment group 1,2 was stronger than the captopril group(P<0.01);6) the effect on TNF-a content:TNF-a contents of normal groups was343.096±18.677pg/ml, model groups was 169.258±14.756 pg/ml, IL-6 contents of treatment group were 381.232±16.415,405.032±28.508,431.564±26.942,469.789±15.007,494.615±21.203 pg/ml. there was significant difference between normal group and other group (P<0.01),showed the model was successful. there was significant difference between model group and treatment groups (P<0.01), the effect of treatment group 1,2was stronger than the captopril group (P<0.01).Conclusion:The Ber had the determinate protective function to the vascular endotheli-al cells Injured by Glucose Insulin and oxidized low-Denslty Lipoprotein,and the function might be realized by advancing NO and inhibiting ET-1,SOD and intercellularadhesion inorder to decrease diabetic vasculopathy.