Analysis Of Healing Efficacy Of Individualized,spcialized Immunal Therapy On Tumor Based On Microenvironment Interference.

Objective:Cytokine-induced killer cells is a new kind of immunologic cells.It is induced by IL-2.IFN-γand so on.It is a kind of cytotoxic lymphocytes which can be used in many kind of tumor treatment.It is named natural killer-like T lymphocytes because of expression of CD3, CD3 monoclonal antibody and CD 56 membrine protein molecules.It can be widely used in clinical treatment because it proliferates quickly ,treats tumor more effectively and the number of the cells can meet our needs in clinical. Dendritic cells are accepted as the most effective antigen-presenting cell.It can provoke T lymphocytes'activation effectively and induce specific immune response.It combines congenital immunity with acquired immunity.So it starts immune response. Antigen specific cytotoxicity T lymphocytes( CTL)is a kind of cells which is activated by mature DC activated by antigen of tumor.It can recognize the antigen of tumor specially,and participate in the specific cell immunal response.This is about our works on this treatment this years.We want to analyze the effects of this treatment on tumor treatment so that we can have a more systemically,completely and deeply known of DC-CIK.Methods: Peripheral blood mononuclear cells were isolated from 59 patients with malignant tumors. The attached cells were induced by TL-4 ,GM-CSF , and TNF-a ,and then confirmed as DCs by flow cytometry ; the suspension cells were induced by CD3 monoclonal antibody ,γ-INF ,and IL-2 .After ten days'cultivation,the number of the cells will achieve 4-5×109 and then confirmed as CIK cells by flow cytometry. Then DCs were injected in drain lymph node hypodermically , while CIK cells were injected in intravenous injection.Results:49 patients accepted this treatment for curing tumor.Their symptoms and manifestation on medical imaging has changed better obviously. Karnofsky Performance Status Score (KPS) has been raised.The quality of life has been improved.The decrease of Treg of 16 patients after interference on micro environment changes significantly(P<0.05).10 patients accepted this treatment after operation.The tumor of 8 patients has been inhibited effectively except 2 patients.Conclusion: Individuallized,specialized immunal therapy on tumor based on microenvironment interference can prevent tumor relapsing after operation,and can delaying the progressing of tumor .It can make the tumor smaller, even disappear.The patients can live better and longer.