The Protective Effect Of Baicalin On Pancreatic Fibrosis In Rats

Objective:To establish the rats model of chronic fibrosing pancreatitis with intraperitoneal injection of DDC and To study the efect and potential mechanism of baicalin on pancreatic fibrotic ratsMethods:Fourty seven male Sprague-Dawley rats were randomly divided into 5 equal groups:normal control group(A group), model control group(B group),low-dose baicalin-treated group (C group), mediate-dose baicalin-treated group(D group), andhigh-dose baicalin-treated group (E group). The rats of the later 4 groups underwent intraperitoneal injectin of 750mg/kg body weight (BW) of DDC twice a week for 4 weeks into the pancreatic duct so as to establish models of pancreatic fibrosis. At the third week the baicalin-treated rats were intraperitoneal injectin with different doses of baicalin(25, 50, and 100 mg/kg body weight) every day, while the normal and model control groups received 0.9% sodium chloride solution instead. lasting 4 weeks, the day befor the last day, the rats were fasted 24 hours and then blood samples were colected from rats'heart. Immediately the rats were put to death and their pancreases were taken out. The levels of hyaluronic acid (HA) and diastase were determined by radioimmunoassay and enzyme dynamia. Pancreatic tissue was embedded by paraffin and cut. The histopathological alterations of pancreatic tissue with HE staining were studied by optical microscopy.immunohistochemistry was used to detect the protein expression of transforming growth factor-β1 (TGF-β1) and CTGF and Fn, and measure the expression of TGF-β1 and Fn and CTGF for the observation by tramission electron microscope. Results:The rats'common conditions of group A,C,D,E,were obviously better than that of model group B. The levels of diastase of the low-dose, mediate-dose, and high-dose baicalin-treated groups were all higher than that of the model control group (P<0.05). The serum level of HA of the low-dose, mediate-dose, and high-dose baicalin-treated groups were all significantly lower than that of the model control group (P< 0.05). Comparison of HA and diastase with the each group used baicalin was nonsense. The degrees of fibrosis of the baicalin-treated groups were ameliorated in comparison with the model control group. The protein content of TGF-β1 and fibronectin (Fn) and CTGF of the low-dose, mediate-dose, and high-dose baicalin-treated groups were all lower than that of the model control group (P<0.05). Comparison with the each group used baicalin was nonsense. Comparison of TGF-β1 and CTGF and Fn with the each group used baicalin was nonsense.Conclusions:The models of chronic pancreatitis rats can be induced with in 4 weeks by intraperitoneal injection of DDC twice a week. Baicalin can improve the common conditions of chronic pancreatitis rats; decrease the levels of HA; improve the level s of amylase; Anti-fibrosis effect of baicalin on pancreatic fibrosis maybe related to its elimination oxygen free radical effect and the content of TGF-β1 and CTGF protein.