Mechanism Of BaoShen 1' Cataplasm On The Treatment Of Yang-Deficiency Syndrome In Chronic Renal Failure

OBJECTIVE:In the pre-clinical research conducted on the basis of experimental study:rats with CRF deficiency syndrome, from the renal function, sex hormones, hemodynamics, renal anemia, renal pathology, the study Acupoint CRF deficiency syndrome The Mechanism; fill Acupoint Application Study blank kidney reached domestic leading level.METHODS:Subjects:weight 200±20g adult SD male rats were 84 healthy, normal control group,12 rats fed the other 72 produced adenine deficiency syndrome in rats with chronic renal failure. Randomly divided into 7 groups, control group, model group, particle group of Niaoduqing, external application of non-acupoint group and combined therapy group A, B, C have been investigated.Research technique:(1)The preparation of baoshen I'ointmentis:baoshen I'oin tmentis based on the aconite, cinnamon, Asarum, clove, pepper, DIO, ginger, etc. by mixing milled powder extract made by the p rocess after adding a certain percentage of pre-prepared Babu M atrix (the main component of gelatin, glycerin, sodium polyacry late, etc.) and add 0.5% penetration enhancers-Azone, a certa in proportion of emulsifiers and preservatives, according to a certain percentage of modulation to a different dose, the same weight cream stand. This issue will kidneys ointment 1 spare se t three dose groups:low dose group acid and 5%(equivalent to delivery by lg/kg),Middle dose group acid and 10%(equivalent to delivery by 2g/kg), high dose group acid and 20%(equivalent to delivery by 4g/kg). Chinese medicine hospital in Hubei Province and by the Nephrology Department of Pharmacy preparation.(2)Experimental technique:Modeling:weight 200±20g total of 84 healthy adult SD male rats, the normal control group,12 rats fed the other 72 produced adenine deficiency syndrome in rats with chronic renal failure. By 200 mg /kg·d dose of adenine made the suspension lml, timed to the rat adenine orally given medicines, the total cumulative dose of 1.5g /only, the entire process of modeling 24d. No. 1-12d, daily administration of 1, the first 12d after the administration the next day. In the first 25d tails blood, measuring BUN, Scr, UA, T, confirmed that the model was successful.Grouping:Randomly divided into 7 groups, control group, model group, particle group of Niaoduqing, external application of non-acupoint group and combined therapy group A, B, C have been investigated. Control group (12 pieces):Only to the false application methods.Model control group (12 pieces):modeling+leave application methods.Granule uremic group (12 pieces):modeling+Niaoduqing Granules.Sticking non-acupoint group (12 pieces):modeling+treatment +Sticking Niaoduqing particles of non-point method (using low-dose external application delivery).Combined therapy group (36 pieces):modeling+Treated+ Niaoduqing Point Application Integrated treatment group A (12 pieces):administration of high dose applicator.Integrated treatment group B (12 pieces):administration of middle'dose applicator.Integrated treatment group C (12 pieces):administration of low dose applicator.The above treatment treatment course is 30 days.①Sticks on the law:The acupuncture point sticks on the law: References from rat Vital, kidneys shu points (considering the anatomical structure is not suitable for rat limb point application, it abandoned complex slip point), with 10% Na2S will Sticking Point and the surrounding skin, rat hair removal 3cm×5cm area, and to the kidneys, and 1 cream coating on the acupuncture points, an area of about 0.5cm×0.5cm, covered with cloth, and fixed with adhesive tape. Each applicator time reached 6-8h,1 day external application, a course of 10 days, a total of three treatments for 30 days. At the same time were the high, medium and low doses kidneys 1 ointment applicator.②Acupoints, and hair removal steps were same, only the use of matrix coating Point, an area of about 0.5cm×0.5cm, covered with cloth, and fixed with adhesive tape. Each applicator time reached 6-8h,1 day Applicator,30 days continuous external application.③Sticking non-point method:The rat abdominal skin, hair removal as described above, the coated low-dose and kidneys, and 1 ointment, an area of about 0.5cm×0.5cm, and covered non-woven, and fixed with adhesive tape. Each applicator time reached 6-8h, 1 day Applicator,30 days continuous external application.Note:The use of drugs or large cloth matrix applicator fixed on the acupuncture points; applicator separated during feeding in rats, lead tape to prevent the rats biting off each other; each applicator reached 6h.④Uremic Clearance Granule:by 2g/kg·d dose of particles made of Niaoduqing suspension 1ml, time to chronic renal failure Kidney-Yang Deficiency Rats were given medicines, from the external application on the first day, a total of 30d.(3)Specimen gathering and processing: Blood plasma:After front makes the mold to succeed and sticks on, end time sticks on the same day, draws blood the law using the stock vein intubation to survey BUN, Scr, UA, T, RBC, HB, NO. Observes the big mouse to appear the red spot and dropsy situation for the medicine spot.(4)Observation examination: ①Daily observation:Including big situations and so on mouse's diet, potable water, body weight, feces and urine, superficial knowledge, active situation, state of mind. The observation of primary mass data big mouse polyuria, drinks, the energetic dispirited, the food are few, the body weight reduces, the wool color is withered, the auricle is pale, item color pale red, narrows the eyes the eye wet, the eyelid edema, the tail cold, the activity reduction, the hair-raising, rolls up, polyuria, around vulva moist and so on kidney deficiency of yang card performance.②renal function:automatic biochemical analyzer serum urea nitrogen:blood urea nitrogen (BUN), serum creatinine (Scr),uric acid(UA).③Kidney ultrastructure:Will keep the big mouse kidney which will take, from the kidney cerebral cortex to the medullary substance vertical selection, by the glutaric dialdehyde, the paraf,ormaldehyde intermixture will do immediately pre-fixedly, after that by 1% tetroxide hungry fixed, further will process, will observe with the light microscope and the electron microscope. Light microscope:The fixed kidney organization-specimen, after the conventional dehydration, the paraffin wax embedding, has carried on the 3um slice under the slicer, HE dyes, observation. Electron microscope:The fixed kidney organization specimen has built the size for 1×1×2mm3 the scrap, washes by PBS 3 times, each time 15 minutes, then with 1% hungry acid fixed 1.5 hours, then wash by PBS 3 times, each time 15 minutes, then with the ethyl alcohol gradient dehydration (50%,70%,80%,90%,95%,100%,100%,100%) each time 15 minutes, by the SPURR low viscosity embedding medicinal preparation percolation embedding (37℃) polymerization (70℃)8 hour above, on the LKB-V slicer, first make half thin slice, after the localization, then makes the ultra thin slice, thickness 50-60nm, fishes has on the Formmer membrane copper screen in the shop, for acetic acid double chlorine glazeafter The lead citrate double dyeing, observes under the H-300 transmission electron microscope.(5)statistics processing all data utilize the medicine statistics software Spssll.0 processing, the measurement material data indicated by the mean value±standard deviation that, uses the T-test; The counting material uses the card side examination; The rank material uses the R analysis. P< 0.05 for has the remarkable difference; P< 0.01 for has the extremely remarkable difference; P> 0.05 is the not remarkable difference.RESULTS:The scientific research result indicated that, tests A, B, C group contrasts around the treatment has the obvious statist ics difference (P< 0.05).Around urinates the poisonous clear gr oup to treat has statistics difference (P<0.05)around, the non-acupuncture point sticks on the group to treat has statistics d ifference (P<0.05), the model control group and the normal grou p do not have the difference (P> 0.05). After tests A, B, C grou p treats sticks on the group contrast with the urine poisonous clear group and the non-acupuncture point to have statistics di fference (P> 0.05), tests A, B, between the C three groups contra sts has statistics difference, A group of curative effects surp ass B group (P<0.05), B group of curative effects surpass C gro up (P<0.05), A group of curative effects surpass C group (P<0.0 5). CONCLUSION:The clinical research indicated that, the acupuncture point sticks on is one kind of non-dialysis easy to do and easy, the curative effect accurate, safe reliable, the price inexpensive, the suitable promotion chronic renal failure method of treatment.This topic plans with the chronic renal failure kidney deficiency of yang card big mouse model, from the blood dynamics, the suppression kidney fibrosis progresses, aspects and so on kidney pathology, studies the acupuncture point to stick on the treatment chronic renal failure the action mechanism; Fills the acupuncture point to stick on the treatment nephrosis experimental study the blank, achieves the domestic leading level. Will build the foundation for this therapy next step development application.