The Effect Of Msi1 Gene Silencing On SGC-7901 Cell Line

Objective To investigate effect of silencing Msi1 gene with siRNA on cell cycle, proliferation, apoptosis, invasion and metastasis of SGC-7901 cell line.Methods Msi1-specific siRNA was designed and synthesized, Small interfering RNA was transfected into SGC-7901 cells to knockdown the Msi1 expression. mRNA levels of Msi1 was detected by RT-PCR, The cell growth inhibition was determined by MTT. The effect of Msi1 siRNA on cell cycle and apoptosis was assayed by flow cytometry ( FCM), protein levels of survivin, caspase3 and mmp9 were detected by Western blot.Results RNA interference efficiently suppressed the Msi1 expression in SGC-7901 cell line. After SGC-7901 cells transfected with Msi1 siRNA, MTT showed proliferation rate was reduced (p<0.05) and Flow cytometry analysis revealed the percentage of apoptotic cells with Msi1siRNA cells(23.36 %±0.75)was higher with control(1.52%±0.46) and liposome-treated control (4.34%±0.98)(p<0.05); Compared with control group, the transfected group had more SGC-7901 cells accumulated at G0/G1 phase, and the S phase was reduced(p<0.05), the expression of survivin, mmp9 protein levels decreased (p<0.05), but the expression of caspase3 was the opposite(p<0.05).Conclusions1. Msi1 expression was obviously suppressed after transfected by Msi1-specific siRNA in SGC7901cells.2. Knockdown of Msi1 by siRNA interference reduced proliferation of SGC7901 cells, inducing apoptosis, G0/G1phase arrested, partly repressed invasion and metastasis in gastric cancer cell line SGC-7901.