| Objective : Research Hospital disinfectants TCCA effervescent, iodophor, glutaraldehyde, hand disinfectants on clinical methicillin-resistant Staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa, Acinetobacter , Enterococcus these five kinds of multi-drug-resistant strains of the disinfection effect,to help us to choose an effective disinfectant for clinical and to prevent the outbreak and spread of hospital infection,so that Protect the environmental safety for medical treatment.At the same time we can maintain medical staff provide the basis for occupational health.Method : Collect 100 clinical common five kinds of multi-drug-resistant strains from the First Affiliated Hospital of Nanchang University: Including methicillin-resistant Staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa, Acinetobacter, Enterococcus strains of the 20 respectively. We go on the test with the corresponding standard strains for quality control strains and we are primarily a study by the following experiment: 1. Determination of qualification test neutralizer common disinfectants ---- TCCA effervescent tablets, iodophor, glutaraldehyde, love good hand disinfectant concentration of neutralizer. 2. With the broth dilution method and sensitivity method we test the four kinds of disinfectant susceptibility minimum inhibitory concentration, minimum bactericidal concentration. 3. With the Suspension quantitative bactericidal test, We measured the above- Mentioned four kinds of different concentrations of disinfectants on the five kinds of common clinical multi-drug-resistant strains and the corresponding standard strains of bacteria at different time rates.Result: 1. Four kinds of disinfectant neutralizer concentration is as follows: TCCA effervescent neutralizer is 0.5% sodium thiosulfate + 0.5% Tween-80; Povidone- iodine neutralizer is 1.0% sodium thiosulfate +1.0% Tween-80; Glutaraldehyde neu- tralizer is 1.0% glycine +0.5% Tween 80; hand disinfectant neutralizer is 2.0% soft phospholipid +2.0% of Tween-80. 2. Broth dilution method measured TCCA effer- vescent / iodophor / glutaraldehyde against methicillin-resistant Staphylococcus au- reus standard strain (ATCC6538) of the MIC, MBC, respectively 500/25/31mg/L and 1000/50/62mg/L; Standard strains of Candida albicans (ATCC10231) of the MIC, MBC, respectively 125/25/31mg/L and 250/50/62mg/L; Standard strains of Pseudo- monas aeruginosa (ATCC15442) of the MIC, MBC, respectively 250/50/31mg/L and 500/100/62mg/L; Pairs of Acinetobacter MIC, MBC, respectively 250/25/31mg/L and 500/50/62mg/L; right of enterococci MIC, MBC, respectively 125/50/62mg/L and 250/100/125mg/L.3 .Sensitivity measured by hand disinfectants good care of the ab- ove five kinds of standard strains of the MIC, MBC in turn are 32/16, 64/32, 32/16, 32/16, 64/32 fold dilution.4. Four kinds of disinfectants against clinical isolates of the 100 drug-resistant strains of MIC, MBC results are as follows: 1) 20 strains of me- -resistant Staphylococcus aureus: To detect the right TCCA-sensitive strains of an eff- ervescent tablet (MIC/ MBC is 250/500), a resistant strain (MIC/ MBC is a 1000/ /2000).The remaining 18 of the MIC, MBC and standard strains were the same,was not detected in other three kinds of disinfectants sensitive strains and resistant strains . 2) 20 strains of Candida albicans: To detect the two pairs of povidone-iodine sen- sitive strains (MIC/ MBC is 12.5/25), 2 strains of resistant strains (MIC/ MBC is a 50/100) and the remaining 16 strains of Candida albicans in MIC/ MBC with the st- andard strains are the same, was not detected in other three kinds of disinfectants sen- sitive strains and resistant strains. 3) 20 strains of Pseudomonas aeruginosa: To detect the right TCCA effervescent-sensitive strains 2 (MIC/ MBC is 125/250) and the remaining 18 of the MIC/ MBC and standard strains were the same; to detect pairs of iodine V-sensitive strain 1 (MIC/ MBC is a 25/50) and the remaining 19 of the MIC/ MBC and standard strains were the same; was not detected in other two kinds of disinfectants sensitive strains and resistant strains. 4) 20 strains of Acinetobacter: To detect resistant strains to glutaraldehyde 1 (MIC, MBC is the 62/125) and the remaining 19 of the MIC, MBC and standard strains were the same; was not detected in other three kinds of disinfectants -sensitive strains and resistant strains. 5) 20 enterococci: the four kinds of disinfectants of the MIC, MBC and standard strains are the same, not detected-sensitive strains and resistant strains. 5. Different concentrations of four kinds of disinfectants on clinical drug-resistant strains of the killing rate is as follows: 1) 250mg / L TCCA effervescent standard strains of Candida albicans and the corresponding 20 clinical drug-resistant strains of the role of 10min each contributed a kill rate of 100%; 500mg / L TCCA effervescent tablets against methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter, Enterococcus standard strains and the corresponding 80 the role of the clinical drugresistant strains of the killing rate of 10 min each contributed 100%. 2) 50 mg / L povidone-iodine against methicillin-resistant Staphylococcus aureus and Candida albicans reference strain and the corresponding 40 the role of the clinical drug-resistant strains of the killing rate of 10 min each contributed 100%; 100 mg / L iodine V on Pseudomonas aeruginosa, Acinetobacter, Enterococcus standard strain and the corresponding 60 the role of the clinical drug-resistant strains of the killing rate of 10 min each contributed 100%. 3) 125mg / L glutaraldehyde and methi- cillin-resistant Staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa, Acinetobacter standard strain and the corresponding 80 the role of the clinical drug- resistant strains of the killing rate of 10 min each contributed 100%; 250mg / L gluta- raldehyde and enterococci standard strain and the corresponding 20 the role of the clinical drug-resistant strains of the killing rate of 10 min each contributed 100%. 4) 1 / 8 Prevention of Cruelty to good hand disinfectants against methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter standard strain and the corresponding 60 the role of the clinical drug-resistant strains of the killing rate of 10 min each contributed 100%; 1 / 16 of Prevention of Cruelty to good hand disinfe- ctants on Candida albicans, Enterococcus standard strain and the corresponding 40 the role of the clinical drug-resistant strains of the killing rate of 10 min each contri- buted 100%. 6. TCCA effervescent tablets, iodophor, glutaraldehyde stock solution of methicillin- Resitant Staphylococcus aureus standard strain of Candida albicans refe- rence strain 100% kill rate of the same duration of action, are respectively, 1 min, 1.5 min, 3 min, and the Prevention of Cruelty to good hand-disinfectant stock solution against methicillin-resistant Staphylococcus aureus standard strain of Candida albi- cans strains of 100% kill rate of the standard role of time was 2 min, 1.5 min.Conclusion: In this study, resistant clinical isolates of 100 detected: 20 strains of me- thicillin-resistant Staphylococcus aureus strains resistant to the TCCA effervescent se- nsitive strains and resistance of a strain of 1, 20 drug-resistant strains of Candida albi- cans strains sensitive to iodophor and the resistance of two strains of 2, 20 drug-resi- stant strains of Pseudomonas aeruginosa TCCA effervescent sensitive strains 2 and iodophor of sensitive strains 1, 20 pairs of glutaraldehyde resistant Acinetobacter str- ains 1, 20 drug-resistant strains of enterococci were not detected in these four kinds of disinfectant resistance of sensitive strains and strains. 2. Common disinfectants ---- TCCA effervescent tablets, iodophor, glutaraldehyde, love good hand disinfectant in the clinical use of the process, if for some reason caused by the concentration of redu- ced, then the appropriate extension of disinfection The role of agent time, also the eff- ect of disinfection can be achieved in order to better control of hospital infection.
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