| Objective: To evaluate the efficacy of cortical bone-derived osteoblasts xenogenic deproteinized bone composites as an alternative to autogenous bone graft in spinal fusion.Methods: (1)We made deproteinized bone derived from calves lower extremity long bone 6-month-old by physical and chemical treatments. (2) Cortical bone slices were obtained from the middle of ulna of a 5-week-old New Zealand white rabbits. By using in vitro tissue culture method, the osteoblast was obtained from the lacunae of osteoprogenitor cell.Collected the second and three passage cells and made into single cell suspension, which were transferred at a sum of 5×106 cells onto xenogeneic deproteinized cancellous bone. They were incubated for 7 days at 37 degrees Celsiusin in a humidified 5 percent carbon-dioxide environment. (3)Adult New Zealand white rabbits(n=60) underwent bilateral lumbar posterolateral fusion procedures and were randomized to receive: I,cortical bone-derived osteoblasts xenogenic deproteined bone composites;II, nongraft;III,xenogenic deproteinized bone;IV,autograft.All animals were sacrificed 10 weeks after surgery to evaluate with gross observation, hands-on mechanics, X-ray, CT and histological examination. The related data were analyzed by statistics.Results: Dased on general observation, hands-on mechanics, X-ray and CT comparison, Group I, IV fusion rate was higher than group II, III and there was statistically different(P<0.05). Group I histological showed the abundant of newly bone formation in contact with the implanted heterogeneous porous bone; II there was no newly bone formation; in group III,there were a small amount of newly bone formation, mostly the fibrous connective tissue; in group IV, there were some newly trabecular bone formation at bone-material interface.Conclusion: The results indicate that cortical bone-derived osteoblasts xenogenic deproteinized bone composites may provide a well substitute for autogenous bone graft in spinal fusion .
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